Muscle Relaxant and Sedative-Hypnotic Activities of Extract of Viola betonicifolia in Animal Models Supported by Its Isolated Compound, 4-Hydroxy Coumarin

Table 7

Effect of 4HC on phenobarbitone-induced sleep.

Treatment	Dose (i.p.)	Onset of sleep (min)	Duration of sleeping (min)
Distilled water	10 mL/kg	25.12 ± 1.25	7.34 ± 2.28
Diazepam	4 mg/kg	5.45 ± 0.08***	56.45 ± 0.00***
4HC	10 mg/kg	26.23 ± 1.34	7.25 ± 1.46
	20 mg/kg	26.54 ± 2.08	7.27 ± 1.76
	30 mg /kg	25.35 ± 1.87	6.24 ± 1.78

Values represent the onset of sleep and duration of sleep (in min) after treatment with distilled water (10 mL/kg), 4HC (10, 20, and 30 mg/kg), or diazepam (4 mg/kg) and then all groups were treated with phenobarbitone (35 mg/kg). Data are presented as mean ± S.E.M (𝑛=6). ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001, all with respect to control.