Poly-β-hydroxybutyrate (PHB) Depolymerase from Fusarium solani Thom

Table 1

Degree of positive or negative effect of independent variables on F. solani Thom PHB depolymerase according to the levels in the Plackett-Burman experimental design.

Variable	Symbol	(−) Level	(0) Level	(+) Level	Main effect	𝑇 value	Significance level
Time (h)	a	48	72	96	0.6667	−3.89	NS
pH	b	4	6	8	0.1422	−0.853	NS
Temperature (^°C)	c	25	30	35	−0.5687	4.32	S
Inoculum size (%, v/v)	d	2	4	6	0.0688	−0.397	NS
PHB (g/L)	e	1	2	3	0	0	NS
Glucose (g/L)	f	5	0	10	0	0	S
Volume/flask (mL)	g	20	50	100	−0.2447	1.89	S
Nitrogen (g/L)	h	0.5	1.0	1.5	−0.02	3.761	S
KH2PO4 (g/L)	i	0.1	0.7	1.3	−0.0512	0.57	NS

Bold type indicates positive effect.
Significant (S); nonsignificant (NS). 𝑡_0.1=1.875; 𝑡_0.05=2.365.