Synthesis and Biological Evaluation of New N,N′-Bis(1-substituted-ethylidene)-ethane1,2-diamine and Their Acetyl and Trifluoroacetyl Derivatives as Cytotoxic and Antimicrobial Agents

Table 4

Cytotoxic effects (LC₅₀; μg/mL)^a of the active compounds (2–5) on some human tumor cell lines using the MTT assay.

Compound no.	Human colon carcinoma HT29	Human hepatocellular carcinoma HepG2	Human breast cancer; MCF 7
2b	41.2	39.4	—
2e	35.8	47.3	51.6
2g	74.3	—^b	—
2h	61.4	47.2	—
2j	40.3	—	54.2
3a	14.6	25.8	32.4
3b	9.6	23.5	3.2
3c	12.9	31.8	9.7
3e	19.1	26.3	34.2
3f	8.4	20.7	2.1
3g	17.9	29.4	28.2
3h	8.8	21.6	6.4
3i	11.8	25.2	30.1
4b	55.1	48.7	64.9
5a	19.8	37.2	46.4
5b	20.5	28.1	48.9
Doxorubicin^c	21.1	1.69	2.14

aLC₅₀: lethal concentration of the compound which causes death of 50% of the cells in 24 h (μg/mL).
bTotally inactive against this cell line. ^cPositive control cytotoxic agent.