Chemical Composition, Antioxidant Potential, and Antibacterial Activity of Essential Oil Cones of Tunisian Cupressus sempervirens

Table 1

Essential oil composition from cones of Cupressus sempervirens.

Number	Volatile compound	RI^a	RI^b	Amount (% of whole EO)	Methods of identification
1	Tricyclene	924	1015	0.14	MS, RI
2	α-Pinene	939	1032	47.51	MS, RI, Co-GLC
3	α-Fenchene	953	1044	0.74	MS, RI
4	Camphene	954	1076	0.15	MS, RI, Co-GLC
5	Sabinene	976	1132	0.6	MS, RI, Co-GLC
6	β-Pinene	980	1118	1.53	MS, RI, Co-GLC
7	β-Myrcene	994	1174	1.28	MS, RI, Co-GLC
8	δ-3-Carene	1011	1159	7.4	MS, RI, Co-GLC
9	α-Terpinene	1018	1188	0.11	MS, RI, Co-GLC
10	p-Cymene	1026	1280	0.2	MS, RI, Co-GLC
11	m-Cymene	1023	1278	1.12	MS, RI, Co-GLC
12	Limonene	1030	1203	1.75	MS, RI, Co-GLC
13	β-Phellandrene	1006	1176	0.13	MS, RI
14	(Z)-b-Ocimene	1040	1246	0.14	MS, RI
15	(E)-b-Ocimene	1050	1266	0.12	MS, RI
16	g-Terpinene	1062	1266	0.12	MS, RI, Co-GLC
17	α,p-Dimethylstyrene	1080	1452	0.15	MS, RI
18	Fenchone	1087	1406	0.13	MS, RI
19	Terpinolene	1088	1290	0.6	MS, RI, Co-GLC
20	Sabinol	1210	1800	0.24	MS, RI
21	Borneol	1165	1719	0.31	MS, RI
22	Terpinen-4-ol	1018	1188	0.97	MS, RI, Co-GLC
23	α-Terpineol	1189	1706	0.17	MS, RI, Co-GLC
24	Carvacrol, methyl ether	1244	1586	0.07	MS, RI, Co-GLC
25	Bornyl acetate	1295	1597	2.75	MS, RI
26	α-Terpinyl acetate	1350	1677	4.11	MS, RI
27	Terpinyl-4 acetate	1333	1709	0.39	MS, RI
28	δ-Elemene	1337	1479	0.24	MS, RI
29	α-Cubebene	1352	1468	0.93	MS, RI
30	α-Ylangene	1372	1493	0.9	MS, RI
31	α-Copaene	1376	1497	1.72	MS, RI, Co-GLC
32	β-Bourbonene	1385	1535	0.15	MS, RI
33	β-Elemene	1391	1600	0.12	MS, RI
34	Longifolene	1413	1575	0.2	MS, RI
35	β-Cubebene	1348	1456	0.69	MS, RI
36	α-Gurjunene	1408	1529	0.37	MS, RI
37	β-Caryophyllene	1415	1612	4.53	MS, RI
38	β-Gurjunene	1432	1612	0.2	MS, RI
39	α-Cedrene	1411	1568	0.14	MS, RI
40	Aromadendrene	1439	1628	0.29	MS, RI
41	α-Humulene	1454	1687	0.64	MS, RI, Co-GLC
42	Alloaromadendrene	1474	1661	0.54	MS, RI
43	Germacrene D	1480	1696	1.14	MS, RI, Co-GLC
44	α-Muurolene	1477	1704	0.15	MS, RI
45	δ-Cadinene	1517	1773	0.11	MS, RI
46	cis-Calamenene	1543	1837	0.16	MS, RI
47	Cadina-1,4-diene	1552	1768	0.12	MS, RI
48	β-Bisabolene	1508	1740	1.73	MS, RI
49	Spathulenol	1576	2144	0.21	MS, RI
50	α-Cedrol	1597	2021	4.99	MS, RI, Co-GLC
51	T-Cadinol	1642	2187	0.25	MS, RI
52	α-Acorenol	1630	2163	0.34	MS, RI
53	β-Acorenol	1637	2212	0.31	MS, RI
54	epi-α-Cadinol	1640	2170	0.11	MS, RI
55	α-Muurolol	1642	2209	0.73	MS, RI
56	β-Eudesmol	1650	2257	0.68	MS, RI
57	α-Eudesmol	1641	2216	0.17	MS, RI
58	α-Cadinol	1649	2255	0.55	MS, RI
59	Cadalene	1674	2200	0.09	MS, RI
60	Sandaracopimara-8(14),15-diene	1960	2255	0.04	MS, RI
61	Manoyl oxide	2010	2396	0.07	MS, RI
62	Isopimara-9-(11),15-diene	1906	2175	0.05	MS, RI
63	Abietadiene	2054	2530	0.05	MS, RI
64	Manool	1989	2376	0.38	MS, RI
65	Kaurene	2048	2399	0.13	MS, RI
66	Totarol	2280	2314	0.31	MS, RI
67	Ferruginol	2295	2330	0.23	MS, RI
				Total (%) 97.69

RI: Retention Index; MS: mass spectrometry; Co-GLC: coinjection.
%: percentage calculated by GC-FID on nonpolar HP-5 capillary column.
^aApolar HP-5 MS column.
^bPolar HP Innowax column.