Application of Food-Grade Proteolytic Enzyme for the Hydrolysis of Regenerated Silk Fibroin from Bombyx mori

Figure 5

SDS-PAGE of the regenerated silk fibroin and its enzymatic hydrolysates obtained using FoodPro in the 1st step and Flavourzyme in the 2nd step. M: molecular weight marker. S-1: regenerated silk fibroin, 84 μg/well. S-2: regenerated silk fibroin, 42 μg/well. S-3: regenerated silk fibroin, 28 μg/well. 1-1: enzymatic hydrolysate obtained using FoodPro at the 1st step, 57 μg/well. 1-2: enzymatic hydrolysate obtained using FoodPro at the 1st step, 29 μg/well. 1-3: enzymatic hydrolysate obtained using FoodPro at the 1st step, 19 μg/well. 2-1: enzymatic hydrolysate obtained using Flavourzyme at the 2nd step, 45 μg/well. 2-2: enzymatic hydrolysate obtained using Flavourzyme at the 2nd step, 23 μg/well. 2-2: enzymatic hydrolysate obtained using Flavourzyme at the 2nd step, 15 μg/well.