Research Article

In Vitro α-Amylase and Protein Glycation Inhibitory Activity of the Aqueous Extract of Flueggea leucopyrus Willd

Figure 2

(a) The overlaid fluorescence emission spectra of the samples after a week that contained (1) BSA + Frc (fructose), (2) BSA + Frc + FLAE (15.6 μg/mL), (3) BSA + Frc + FLAE (31.25 μg/mL, (4) BSA + Frc + FLAE (250 μg/mL), (5) BSA + Frc + FLAE (125 μg/mL), (6) BSA + Frc + FLAE (62.5 μg/mL), (7) BSA only, (8) Frc only. (b) Comparison of percentage inhibition of fluorescent AGEs by FLAE and aminoguanidine. For concentrations at 62.5 µg/mL and above, FLAE showed an average percentage inhibition higher than 50%. At concentrations of 62.5 µg/mL and below, FLAE showed a significantly lower activity than aminoguanidine at 250 µg/mL. However, at 250 µg/mL concentration, FLAE showed a significantly higher activity compared to aminoguanidine at the same concentration. between %I of FLAE and I% of AG at 250 µg/mL. The results are presented as mean ± standard deviation of three independent experiments. In certain cases, error bars cannot be visualized due to the values being extensively small.
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