Evidence for the Formation of Ozone (or Ozone-Like Oxidants) by the Reaction of Singlet Oxygen with Amino Acids

Figure 1

HPLC chromatograms of vinylbenzoic acid (a), 4-carboxybenzaldehyde (b), and the reaction mixture obtained by incubating vinylbenzoic acid and methionine sulfoxide with a singlet oxygen-generating myeloperoxidase system (c), showing some conversion of vinylbenzoic acid to 4-carboxybenzaldehyde. Incubating methionine with vinylbenzoic acid and the myeloperoxidase system gave a similar chromatogram.

(a)

(b)

(c)