Laccase Inhibition by Mercury: Kinetics, Inhibition Mechanism, and Preliminary Application in the Spectrophotometric Quantification of Mercury Ions

Table 1

Kinetic constants for different inhibition times of TvL by mercury(II), monitoring the CAQ absorbance at 410 nm in acetate buffer ().

Inhibition time (minutes)	Km (μM)	Vmax (μM·s^−1)	Kcat (μMmL·μg^−1)	kcat/Km (mL·μg^−1)
0	43 ± 4	90 ± 3	9.0 ± 0.3	0.28 ± 0.03
2	55 ± 5	53 ± 2	5.3 ± 0.2	0.10 ± 0.01
5	58 ± 7	51 ± 3	5.1 ± 0.3	0.09 ± 0.04
10	57 ± 5	56 ± 3	5.6 ± 0.3	0.10 ± 0.03