Laccase Inhibition by Mercury: Kinetics, Inhibition Mechanism, and Preliminary Application in the Spectrophotometric Quantification of Mercury Ions

Table 4

Indirect analytical methods for quantification of Hg(II) found in the literature that are based on the principle of enzymatic inhibition.

	Detection method	LOD (ppm)	Linear interval (ppm)	pH	Incubation time (min)	Comment	Ref.
Invertase biosensor	Amperometric	0.001	0.01–0.04	0.1 M NaOH	10	Biosensor coupled to a batch injection analysis	[32]
Invertase immobilized	Thermometric	0.005	0.005–0.08	7.0	2–6	Coupling to FIA	[33]
GOx biosensor	Amperometric	0.05	2–32	7.0	—	Nafion- and MnO2-modified CPE	[34]
Invertase-mutarotase-GOx biosensor	Conductimetric	0.005	—	6.5	10–20	—	[35]
GOx free	Colorimetric	0.015	0.005–1	5.0	2	—	[36]
Alcohol ox. and horseradish peroxidase free	Fluorescence	2.5 × 10^−6	5.0 × 10^−6–0.05	7.5	20	—	[37]
Sucrose biosensor	Amperometric	—	0.002–0.2	6.0	20	—	[38]
GOx biosensor	Amperometric	—	—	7.0	30	—	[39]
GOx and laccase biosensor	Amperometric	0.03	—	5.0	—	—	[40]
GOx	Amperometric	0.5	0.4–36	7.0	—	—	[41]
Laccase free	Colorimetric	15	10–120	4.5	2	—	This work