A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

Figure 8

The optimal conditions of the immunoassay. (a) Blocking reagent. (b) Incubation temperature during blocking step. (c) DMSO concentration in PBS buffer. (d) SA-HRP concentration. (e) Incubation time. (f) Ion concentration of PBS. (g) pH of PBS.

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(b)

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