A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

Table 3

The optimization of coating condition for OVA-BaP.

Coating condition		IC50 (μg L^−1)
4°C overnight	1.07	1.73	0.62
25°C
1 h	0.76	2.39	0.32
2 h	0.81	2.21	0.37
37°C
1 h	0.92	2.26	0.41
2 h	1.05	1.78	0.59
43°C
1 h	0.72	2.51	0.29
2 h	0.63	3.37	0.19