A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis

Table 4

Optimal concentrations of OVA-BaP and bio-pAb-BaP.

Dilutions of bio-pAb-BaP	OVA-BaP concentration (µg mL^−1)
	28.3	14.15	5.66	2.83	1.42	0.71
100	2.855	2.166	2.011	1.972	2.244	2.612
500	1.997	1.657	1.721	1.851	1.879	2.446
1000	1.275	1.419	1.396	1.367	1.208	1.468
2000	0.865	1.036	1.039	0.910	0.820	0.907
4000	0.393	0.750	0.902	0.490	0.392	0.636
Blank	0.136	0.129	0.167	0.159	0.137	0.162

initial concentration of the prepared OVA-BaP was 2.83 mg mL⁻¹; the OVA-BaP was diluted with PBS buffer. initial concentration of the prepared bio-pAb-BaP was 0.985 mg mL⁻¹; the bio-pAb-BaP was diluted with CBS buffer.