Research Article

Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer

Table 2

Peak assignments for SERS spectra of serum.

Raman shift (cm−1)Peak assignment

630Glycerol
642C-C twisting mode of tyrosine
678Ring breathing modes in the DNA bases
728C-C stretching, proline
787Uracil-based ring breathing mode
817C-C stretching (protein)
829O-P-O stretching DNA/RNA; ν2PO2− stretch of nucleic acids
855Proline, hydroxyproline, and tyrosine C-C stretching
880Tryptophan, δ (ring)
921C-C stretch of proline ring/glucose/lactic acid; C-C, praline ring (collagen assignment)
960Hydroxyapatite, carotenoid, and cholesterol
1005Phenylalanine (proteins); proteins symmetric ring breathing of phenylalanine
1017Carbohydrates peak
1031Phenylalanine, C-N stretching of proteins; C-H in-plane bending mode of phenylalanine
1047Glycogen
1079C-C or C-O stretching mode of phospholipids
1120The strong C-O band of ribose
1143C-O stretching (carbohydrates)
1171Tyrosine; (CH) phenylalanine, tyrosine
1210C-C6H5 stretching mode in tyrosine and phenylalanine
1255Lipids
1270Typical phospholipids, C=C groups in fatty acids, amide III band in proteins
1318G (ring breathing modes of the DNA/RNA bases)
1335Polynucleotide chain (DNA purine bases)
1379δCH3 symmetric (lipid assignment)
1425G, A (DNA, RNA)
1446CH2 bending mode of proteins and lipids; CH2 deformation
1462δCH2, disaccharides
1488Guanine (N7)
1534Amide carbonyl group vibrations and aromatic hydrogens
1565Tryptophan
1583C=C bending mode of phenylalanine
1601Amide I band of proteins
1641Amide I band (protein band)
1673Amide I

Raman peaks assignments are based on Ref. [2537].