Chemical Constituents from the Roots of Polygala arillata and Their Anti-Inflammatory Activities

Figure 5

Anti-inflammatory effects of the isolated compounds and inhibition of LPS-induced NO production in RAW 264.7 mouse macrophages. The cells were pretreated with concentrations (12.5, 25, and 50 μg/mL) of compounds (1, 2, 4, 5, 7, 8, and 10) or C₂₂H₂₈FNa₂O₈P (5 μg/mL) for 1 h and then stimulated with LPS (0.1 μg/mL) for 16 h. The data show the mean ± SD of three independent experiments performed in triplicates. and compared to the LPS-treated values. Arillatanoside E (1), oleanolic acid (2), trans-ferulic acid (4), trans-feruloyl-glucoside (5), 2,4,6-trimethoxy-1-O-β-D-glycoside (7), 3-methoxy-4-hydroxybenzoic acid (8), and sinapic acid (10).