Anti-Inflammatory Compounds from Vietnamese Piper bavinum

Figure 4

Inhibition of LPS-induced iNOS expression in RAW 264.7 cells by compound 4 (C4). RAW 264.7 cells were pretreated for 30 min at indicated concentrations of 0, 3, 10, and 30 μM, followed by stimulation with LPS (1 μg/mL) for 24 h. Whole-cell lysates were blotted with the indicated antibodies. α-tubulin level was used as a loading control. The expression levels of iNOS and COX-2 were determined by the analysis of immunoblot.