Journal of Chemistry

Research Article

The Research and Mechanism of Extracting Vitamin B6 Using Aqueous Two-Phase Systems

Table 2

Binodal data for the mixture of polyethylene glycol/isopropyl alcohol + ethanol + salt (Na₂CO₃/Na₂SO₄) + water ATPSs at different temperatures^a.


100W₁	100W₂	100W₁	100W₂	100W₁	100W₂	100W₁	100W₂	100W₁	100W₂

Polyethylene glycol + ethanol + Na₂SO₄ + H₂O system at T = 308.15 K MA₁ = 0.200
46.51	2.20	30.59	5.47	27.23	6.67	20.13	9.97	8.57	16.79
44.43	2.47	30.43	5.55	27.21	6.72	19.08	10.49	8.09	17.13
43.01	2.57	30.42	5.60	26.71	6.87	18.02	11.04	7.72	17.47
42.09	2.69	30.22	5.60	26.67	6.93	17.06	11.55	7.23	17.88
40.94	2.96	29.99	5.66	26.61	6.98	16.02	12.14	6.98	18.25
39.56	3.19	29.79	5.70	26.27	7.07	15.35	12.46	6.35	18.94
37.97	3.56	29.55	5.81	26.20	7.13	14.52	12.93	5.57	19.77
36.56	3.90	29.19	5.91	25.90	7.22	13.73	13.33	4.19	20.71
35.18	4.22	28.91	6.02	25.77	7.29	13.07	13.72	3.50	21.84
33.92	4.52	28.49	6.13	25.55	7.37	12.55	13.98	2.09	24.05
32.87	4.72	28.25	6.19	25.48	7.43	12.02	14.29
32.60	4.81	28.22	6.27	25.37	7.50	11.52	14.57
31.90	5.03	28.23	6.31	25.19	7.61	11.16	14.88
31.64	5.13	28.19	6.35	24.94	7.67	10.70	15.15
31.43	5.21	28.08	6.41	24.93	7.74	10.45	15.44
31.26	5.29	27.81	6.48	23.80	8.22	9.92	15.73
30.98	5.34	27.57	6.55	22.32	8.95	9.34	16.11
30.87	5.41	27.49	6.60	21.30	9.39	9.14	16.44

Polyethylene glycol + ethanol + Na₂SO₄ + H₂O system at T = 318.15 K MA₁ = 0.200
43.94	2.92	24.72	8.09	19.61	10.50	14.07	13.24	7.93	16.99
40.39	3.46	24.33	8.23	19.18	10.65	13.76	13.41	7.46	17.25
38.40	3.84	24.10	8.37	18.83	10.86	13.48	13.61	6.96	17.53
37.43	4.04	23.99	8.55	18.68	10.98	13.15	13.79	6.64	18.05
35.11	4.51	23.22	8.70	18.47	11.11	12.88	13.99	6.07	18.38
34.08	4.81	22.41	9.09	17.98	11.27	12.54	14.20	5.55	18.71
33.11	5.07	22.29	9.17	17.35	11.64	12.17	14.43	5.08	19.07
30.79	5.68	22.01	9.33	17.14	11.76	11.50	14.70	4.49	19.73
30.39	5.83	21.53	9.53	16.94	11.91	11.16	14.91	4.09	20.19
30.14	5.92	21.01	9.77	16.33	12.08	10.79	15.13	3.59	21.16
29.33	6.25	20.85	9.86	16.10	12.23	10.46	15.36	2.89	22.12
28.39	6.56	20.72	9.95	15.83	12.39	10.05	15.59	2.32	23.99
27.27	7.02	20.30	10.08	15.56	12.54	9.71	15.87
26.19	7.54	20.08	10.18	15.26	12.70	9.31	16.11
25.47	7.86	19.93	10.28	14.99	12.84	8.83	16.44
25.02	7.92	19.76	10.38	14.75	13.01	8.38	16.71

Polyethylene glycol + ethanol + Na₂SO₄ + H₂O system at T = 328.15 K MA₁ = 0.200
47.94	2.12	26.00	7.69	14.22	13.32	10.15	15.42	7.64	16.92
43.23	2.84	24.08	8.53	13.40	13.71	9.91	15.55	7.28	17.15
39.13	3.67	22.11	9.46	12.43	14.23	9.82	15.57	6.92	17.40
36.53	4.18	20.40	10.27	12.20	14.38	9.56	15.78	6.41	17.77
34.30	4.85	18.66	11.14	11.61	14.66	8.96	16.14	5.95	18.12
31.82	5.74	17.51	11.68	11.01	14.97	8.76	16.22	5.15	18.84
29.85	6.23	16.32	12.26	10.47	15.25	8.42	16.44	4.54	19.35
28.06	6.86	15.31	12.75	10.31	15.35	8.06	16.67	2.19	21.98

Isopropyl alcohol + ethanol + Na₂SO₄ + H₂O system at T = 308.15 K MA₂ = 0.333
48.25	0.96	30.49	5.06	26.14	7.04	21.16	9.91	16.05	13.62
43.37	1.65	30.36	5.10	25.98	7.11	21.09	10.01	15.97	13.78
37.89	2.84	30.32	5.16	25.90	7.19	20.79	10.12	15.52	14.11
36.03	3.23	29.97	5.23	25.72	7.25	20.75	10.23	15.43	14.35
35.90	3.31	29.85	5.32	25.55	7.33	20.65	10.32	14.83	14.60
35.48	3.40	29.52	5.39	25.47	7.41	20.57	10.41	14.67	14.80
35.14	3.49	29.31	5.45	25.47	7.46	20.27	10.57	14.41	15.00
34.99	3.54	29.35	5.50	25.24	7.51	20.15	10.67	14.15	15.19
34.80	3.59	29.42	5.54	25.22	7.56	20.04	10.76	13.89	15.39
34.60	3.65	29.32	5.60	25.10	7.64	19.88	10.87	13.67	15.61
34.47	3.71	29.18	5.65	24.99	7.72	19.65	10.97	13.49	15.85
34.25	3.77	28.80	5.74	24.62	7.95	19.52	11.06	13.19	16.19
34.09	3.82	28.74	5.79	24.53	8.06	19.34	11.17	12.41	16.68
33.71	3.92	28.59	5.86	23.90	8.33	19.16	11.27	11.81	17.19
33.55	3.99	28.32	5.93	23.85	8.39	19.03	11.38	11.15	17.70
33.03	4.08	28.30	5.98	23.76	8.50	18.86	11.49	10.89	18.15
32.83	4.16	28.29	6.04	23.53	8.58	18.71	11.60	10.27	18.41
32.39	4.27	28.12	6.10	23.54	8.66	18.61	11.74	9.78	18.70
32.30	4.34	28.02	6.17	23.30	8.76	18.48	11.85	9.52	19.16
32.08	4.40	27.83	6.24	23.16	8.88	18.39	11.95	9.08	19.46
31.82	4.51	27.77	6.30	23.09	8.95	18.05	12.18	8.77	19.94
31.52	4.57	27.60	6.35	22.92	9.06	17.86	12.33	8.34	20.28
31.53	4.64	27.37	6.49	22.56	9.21	17.76	12.44	8.01	20.82
31.59	4.68	27.19	6.58	21.89	9.43	17.52	12.58	7.55	21.39
31.47	4.75	26.98	6.67	21.85	9.51	17.38	12.76	7.17	21.93
31.11	4.82	26.77	6.76	21.77	9.58	17.02	13.02	6.04	23.12
31.07	4.86	26.61	6.80	21.67	9.65	16.83	13.14
30.97	4.92	26.61	6.84	21.57	9.71	16.44	13.32
30.64	5.02	26.31	6.97	21.24	9.83	16.21	13.49

Isopropyl alcohol + ethanol + Na₂CO₃ + H₂O system at T = 308.15 K MA₂ = 0.333
33.98	3.28	28.36	5.03	24.68	6.66	20.35	8.65	16.52	10.98
33.68	3.34	28.21	5.12	24.35	6.81	20.29	8.72	16.38	11.08
33.46	3.42	27.80	5.25	24.04	6.96	20.19	8.79	16.22	11.20
33.12	3.50	27.54	5.34	23.75	7.07	20.07	8.87	16.05	11.31
32.90	3.58	27.35	5.44	23.52	7.22	19.92	8.94	15.65	11.55
32.64	3.64	27.08	5.57	23.18	7.33	19.66	9.10	15.20	11.82
32.41	3.72	26.81	5.68	22.80	7.46	19.31	9.20	14.81	12.09
32.08	3.80	26.57	5.75	22.65	7.57	19.19	9.28	13.69	12.92
31.72	3.92	26.38	5.82	22.08	7.70	19.08	9.36	12.55	13.42
31.36	4.02	26.19	5.90	21.91	7.81	18.95	9.44	11.74	13.97
31.10	4.09	26.06	6.00	21.77	7.94	18.70	9.62	10.59	14.90
30.59	4.25	25.82	6.08	21.55	8.02	18.40	9.80	9.66	15.67
30.44	4.36	25.72	6.15	21.43	8.14	17.98	9.92	8.11	16.86
29.96	4.47	25.50	6.23	21.28	8.27	17.71	10.11	7.01	18.30
29.81	4.57	25.27	6.32	20.98	8.35	17.44	10.30	5.27	21.03
29.45	4.66	25.18	6.40	20.91	8.42	17.16	10.53
29.21	4.78	25.08	6.48	20.78	8.49	17.06	10.72
28.76	4.92	24.81	6.57	20.66	8.56	16.63	10.86

^aStandard uncertainties u are u (T) = 0.1 K and u (p) = 10 kPa, and the average uncertainty of u (w) = 0.01.