Journal of Chemistry

Research Article

The Research and Mechanism of Extracting Vitamin B6 Using Aqueous Two-Phase Systems

Tie-line data for the mixture of polyethylene glycol/isopropyl alcohol + ethanol + salt (Na₂CO₃/Na₂SO₄) + water ATPSs at different temperatures^a.


T (K)	Total system		Top phase		Bottom phase		TLL	S
T (K)	100W₁	100W₂	100W₁	100W₂	100W₁	100W₂	TLL	S

Polyethylene glycol + ethanol + Na₂SO₄ + H₂O system
308.15	19.53	11.57	27.35	6.65	4.01	21.33	18.33	−1.59
	14.00	14.18	23.54	8.18	5.78	19.35	9.71	−1.59
	15.00	13.85	24.99	7.57	5.06	20.11	11.75	−1.59
	16.49	12.79	24.41	7.81	5.34	19.80	13.17	−1.59
	17.98	12.16	25.78	7.25	4.69	20.52	15.70	−1.59

318.15	20.89	11.94	31.17	5.79	2.27	23.07	21.70	−1.67
	17.20	12.76	25.32	7.90	4.04	20.64	15.34	−1.67
	18.79	12.22	27.26	7.15	3.36	21.46	17.99	−1.67
	16.54	12.91	24.04	8.42	4.53	20.10	14.00	−1.67
	18.00	12.36	25.70	7.75	3.90	20.80	16.43	−1.67

328.15	28.74	7.34	31.83	5.73	3.84	20.35	28.09	−1.91
	26.25	8.39	30.50	6.16	4.27	19.89	24.81	−1.91
	17.34	12.44	26.72	7.53	5.80	18.48	13.03	−1.91
	22.11	10.17	28.24	6.96	5.15	19.05	15.09	−1.91
	18.63	11.95	27.97	7.06	5.26	18.95	11.89	−1.91
	15.60	13.60	28.51	6.90	5.07	19.12

Isopropyl alcohol + ethanol + Na₂SO₄ + H₂O system
308.15	22.86	10.01	27.53	6.39	6.35	22.81	20.89	−1.29
	18.90	12.46	25.21	7.57	8.00	20.91	13.79	−1.29
	17.30	13.58	24.68	7.86	8.40	20.48	11.26	−1.29
	24.58	8.79	27.89	6.22	6.11	23.11	23.37	−1.29
	19.56	12.12	25.91	7.20	7.47	21.49	15.30	−1.29

Polyethylene glycol + ethanol + Na₂CO₃ + H₂O system
308.15	17.61	11.07	28.05	5.17	10.81	14.91	7.81	−1.77
	17.86	11.15	29.15	4.77	9.83	15.69	9.22	−1.77
	18.11	11.20	30.02	4.47	9.08	16.30	10.37	−1.77
	18.45	11.28	31.16	4.10	8.17	17.09	11.81	−1.77
	18.77	10.96	30.60	4.28	8.63	16.69	11.65	−1.77

^aStandard uncertainties u are u (T) = 0.1 K and u () = 10 kPa, and the average uncertainty of u () = 0.01.