SERS Platform Based on Bimetallic Au-Ag Nanowires-Decorated Filter Paper for Rapid Detection of miR-196ain Lung Cancer Patients Serum

Figure 4

(a) SEM images of the AgNW@AuNPs substrate. (b) SERS mapping of NBA measured at 592 cm⁻¹ using the AgNW@AuNPs substrate. (c) SERS spectrum of the AgNW@AuNPs substrate labeled DNA hairpin probe. (d) Assembly time-dependent SERS intensity at 1334 cm⁻¹. (e) Time change of SERS intensity for the complementary miRNA (blue triangle), single-base mismatch miRNA (red circle), and noncomplementary miRNA (black square). (f) SERS spectra of SERS platform before (original) and after incubation with 100 pM complementary, noncomplementary, and single-base mismatch miRNA. (g) SERS spectra at 1334 cm⁻¹ from 6 random spots of the SERS platform after hybridization with 100 pM miR-196a. (h) The line chart of the intensity at 1334 cm⁻¹ corresponding to the SERS spectra in (g). (i) The reproducibility of the SERS platform. (j) The histogram of the intensity at 1334 cm⁻¹ corresponding to the SERS spectra in (I).

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