Bioactivity-Guided Single-Step Isolation of Stachyspinoside from Sideritis congesta by Centrifugal Partition Chromatography

Figure 3

HPLC chromatograms of the aqueous crude extracts obtained from S. congesta with a charged aerosol detector (CAD) (a) and PDA detector at 260 nm (b). The peak at 21.6 min corresponds to stachyspinoside (1), and the peak at 18.1 min (2) corresponds to the highest closest peak to stachyspinoside and was considered for the calculation of the separation factor .

(a)

(b)