Bioactivity-Guided Single-Step Isolation of Stachyspinoside from Sideritis congesta by Centrifugal Partition Chromatography

Figure 4

CPC separation of stachyspinoside 1 (peak at 28.5 minutes) performed in the ascending mode (detection at 260 nm). Solvent system: terAcWat +1.5_0.1%AA; sample concentration 6 mg/mL; detection wavelength 260 nm; flow rate: 50 mL/min; rotor speed: 1500 rpm; and retention of stationary phase: 55.1%.