Bioactivity-Guided Single-Step Isolation of Stachyspinoside from Sideritis congesta by Centrifugal Partition Chromatography

Figure 5

(a) Chromatogram of CPC fractionation of the aqueous extract from S. congesta in descending mode. Solvent system: terAcWat +1; sample concentration 2 mg/mL; detection wavelength 260 nm; flow rate: 30 mL/min; rotor speed: 1500 rpm; retention of stationary phase: 62.5%; retention time of fraction I (8–12 min); and retention time of fraction II (18–26 min). (b) Chromatogram of CPC fractionation of the aqueous extract from S. congesta in ascending mode. Solvent system: terAcWat +1; sample concentration 2 mg/mL; detection wavelength 260 nm; flow rate: 30 mL/min; rotor speed: 1500 rpm; retention of stationary phase: 70.5%; and retention time of fraction III (1.5–15 min).

(a)

(b)