Bioactivity-Guided Single-Step Isolation of Stachyspinoside from Sideritis congesta by Centrifugal Partition Chromatography

Figure 7

(a) An example HPLC-CAD chromatogram of fraction III used for the quantification of peaks 1–5 with caffeine as internal standard. (b) HPLC-PDA chromatogram of preparative separation of fraction III. The collection of the peaks 1–5 was performed with a fraction collector Foxy R1 (KNAUER) at 200 nm. The obtained solutions were evaporated, and the dried residues were used for the structure identification via HRMS and NMR.

(a)

(b)