Bioactivity-Guided Single-Step Isolation of Stachyspinoside from Sideritis congesta by Centrifugal Partition Chromatography

Table 1

Partition coefficients (K) and separation factor () of stachyspinoside 1 in various solvent systems.

Solvent system	Partition coefficient (K)		Separation factor ()
	Stachyspinoside (K1)	Peak 2 (K2)
terAcWat +1	0.88	0.71	1.2
terAcWat +1.5	0.58	0.44	1.3
terAcWat +1.5_0.1%AA	0.50	0.23	2.2

The results are determined in accordance with chapter 2.5 and correspond to the descending mode. AA = ammonium acetate. Peak 2 corresponds to the highest closest peak to stachyspinoside (retention time 18.1 min).  = K₁/K₂, K₁> K₂.