Antimicrobial and Antibiofilm Activities of 4,5-Dihydro-1H-pyrazole-1-carboximidamide Hydrochloride against Salmonella spp.

Table 1

Revertants per plate (mean and standard deviation) of S. typhimurium TA98 and TA100 strains after treatment with the (trifluoromethyl)phenyl-substituted compound in the presence and absence of metabolic activation.

Treatment (µg.plate-1)	TA98		TA100
	−S9	+S9	−S9	+S9
0.0	11 ± 1	13 ± 2	73 ± 9	80 ± 13
50	11 ± 3	15 ± 1	76 ± 7	91 ± 6
150	11 ± 1	10 ± 3	80 ± 8	89 ± 3
500	13 ± 2	12 ± 5	79 ± 10	103 ± 10
1500	8 ± 1	11 ± 5	68 ± 10	91 ± 7
5000	3 ± 1	7 ± 2	54 ± 4	9 ± 1
C+	295 ± 6^a	306 ± 8^b	912 ± 5^c	987 ± 9^b

0.0: negative control: dimethyl sulfoxide (DMSO); positive controls: 4-nitrophenylenediamine (10 μg.plate⁻¹)^a; 2-AA aminoanthracene (0.625 μg.plate⁻¹)^b; sodium azide (2.5 μg.plate⁻¹)^c.