New Purinyl-Steroid and Other Constituents from the Marine Fungus Penicillium brefeldianum ABC190807: Larvicidal Activities against Aedes aegypti

Table 1

¹H-NMR (400 MHz) and ¹³C-NMR (101 MHz) data of compound 1 in CDCl₃ (δ in ppm, J in Hz).

Position	δ(H)	δ(C)	HMBC
1α	1.32 (td, 14.4, 3.2)	29.4 CH2
1β	1.67–1.60 (m)
2α	2.15–1.99 (m)	25.5 CH2
2β	2.24 (tdd, 14.4, 5.2, 3.3)
3	5.32 (dd, 4.8, 5.2)	48.9 CH	C-8′, 1, 5 ′, 1, 5
4	5.56 (d, 4.8)	116.6 CH	C-2, 10, 6
5		150.2 C
6	6.00 (d, 9.7)	124.7 CH
7	6.35 (d, 9.7)	128.1 CH
8		124.2 C
9	2.13–2.05 (m)	45.3 CH
10		36.2 C
11	1.66–1.53 (m)	19.5 CH2
12α	1.39–1.17 (m)	35.9 CH2
12β	2.15–1.99 (m)
13		43.8 C
14		151.7 C
15	2.57–2.31 (m)	25.1 CH2
16α	1.50–1.43 (m)	27.8 CH2
16β	1.86–1.74 (m)
17	1.39–1.17 (m)	55.9 CH
18	0.94 (s)	17.6 CH3	C-1, 5, 9
19	0.95 (s)	19.1 CH3	C-12,14, 17
20	2.23–2.10 (m)	39.3 CH
21	1.05 (d, 6.7)	21.2 CH3	C-17, 22
22	5.20 (dd, 15.3, 7.7)	135.2 CH
23	5.25 (dd, 15.3, 7.1)	132.3 CH
24	1.94–1.83 (m)	42.9 CH
25	1.52–1.43 (m)	33.1 CH
26	0.83 (d, 6.7)	19.6 CH3	C-24
27	0.93 (d, 6.7)	17.6 CH3	C-23, 25
28	0.85 (d, 6.7)	20.0 CH3	C-24
2′’	8.38 (s)	152.6 CH	C-6′, 4′, 4′
4′		149.4 C
5′		120.2 C
6′		155.3 C
8′	7.79 (s)	140.2 CH	C- 4′, 5′, 5′
6′-NH2-NH2	5.83 (s)