Journal of Chemistry

Research Article

TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

Table 1

Detailed information regarding upregulated and downregulated differentially expressed proteins in S. putrefaciens cultivated at 4°C and 30°C.


Accession¹	Sequence length	FC²	Gene names	Locus	Protein names

Amino acid transport and metabolism
A4Y849	505	0.52		Sputcn32_2411	L-Lysine 6-monooxygenase, EC 1.14.13.59
A4Y315	797	0.61		Sputcn32_0617	Bifunctional aspartokinase/homoserine dehydrogenase (includes aspartokinase, EC 2.7.2.4; homoserine dehydrogenase, EC 1.1.1.3)
A4Y550	456	0.70		Sputcn32_1355	Zinc metalloprotease, EC 3.4.24.-
A4Y4D8	451	0.72		Sputcn32_1093	L-Glutamine synthetase, EC 6.3.1.2
A4YC19	582	0.80		Sputcn32_3795	Urocanate reductase, EC 1.3.99.33
A4Y7I3	1614	0.82		Sputcn32_2195	Glutamate dehydrogenase, EC 1.4.1.2
A4Y7Q7	939	1.20		Sputcn32_2269	2-Oxoglutarate dehydrogenase E1 component, EC 1.2.4.2
A4Y4E5	425	1.22		Sputcn32_1100	4-Aminobutyrate aminotransferase, EC 2.6.1.19
A4Y656	234	1.28		Sputcn32_1714	3-Oxoacid CoA-transferase, A subunit, EC 2.8.3.5
A4Y1J0	679	1.31		Sputcn32_0087	Oligopeptidase A, EC 3.4.24.70
A4Y3F6	358	1.32	alr	Sputcn32_0759	Alanine racemase, EC 5.1.1.1
A4Y661	389	1.35		Sputcn32_1719	Isovaleryl-CoA dehydrogenase, EC 1.3.8.4
A4Y4D3	285	1.39		Sputcn32_1088	3-Mercaptopyruvate sulfurtransferase, EC 2.8.1.2
A4Y657	315	1.42		Sputcn32_1715	Hydroxymethylglutaryl-CoA lyase, EC 4.1.3.4
A4Y3U2	189	1.43		Sputcn32_0896	Alkyl hydroperoxide reductase C, EC 1.11.1.15
A4Y2I9	524	1.44		Sputcn32_0440	Histidine ammonia-lyase, EC 4.3.1.3
A4Y540	274	1.45	dapD	Sputcn32_1345	2,3,4,5-Tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, EC 2.3.1.117
A4Y542	278	1.45	map	Sputcn32_1347	Methionine aminopeptidase, EC 3.4.11.18
A4YBI5	377	1.46		Sputcn32_3610	Alanine-glyoxylate aminotransferase, EC 2.6.1.44
A4Y342	405	1.46	argD	Sputcn32_0645	Acetylornithine aminotransferase, EC 2.6.1.11
A4YCC5	341	1.47	tdh	Sputcn32_3902	L-Threonine 3-dehydrogenase, EC 1.1.1.103
A4Y3C6	386	1.54		Sputcn32_0729	Methionine gamma-lyase, EC 4.4.1.11
A4Y6M4	344	1.55	astE	Sputcn32_1884	Succinylglutamate desuccinylase, EC 3.5.1.96
A4YCC4	397	1.56	kbl	Sputcn32_3901	2-Amino-3-ketobutyrate coenzyme A ligase, EC 2.3.1.29
A4Y7N9	444	1.61	astB	Sputcn32_2251	N-succinylarginine dihydrolase, EC 3.5.3.23
A4Y1I2	555	1.82	hutU	Sputcn32_0079	Urocanate hydratase, EC 4.2.1.49
A4Y1I0	408	1.85	hutI	Sputcn32_0077	Imidazolonepropionase, EC 3.5.2.7
A4Y1I7	451	1.89		Sputcn32_0084	NADPH-glutathione reductase, EC 1.8.1.7
A4Y596	300	1.89		Sputcn32_1402	3-Hydroxyisobutyrate dehydrogenase, EC 1.1.1.31
A4YAE0	364	1.93	gcvT	Sputcn32_3211	Aminomethyltransferase, EC 2.1.2.10
A4Y612	346	2.05		Sputcn32_1670	4-Hydroxyphenylpyruvate dioxygenase, EC 1.13.11.27
A4Y659	288	2.09		Sputcn32_1717	Methylglutaconyl-CoA hydratase, EC 4.2.1.18
A4Y8W2	197	2.18		Sputcn32_2676	Thiol peroxidase, EC 1.11.1.15
A4Y946	370	2.20	aguA	Sputcn32_2760	Putative agmatine deiminase, EC 3.5.3.12
A4Y3Y4	169	2.21	luxS	Sputcn32_0938	S-Ribosylhomocysteine lyase, EC 4.4.1.21
A4Y733	396	2.26		Sputcn32_2045	Aminotransferase, EC 2.6.1.-
A4YAD9	129	2.31	gcvH	Sputcn32_3210	Glycine cleavage system H protein

Carbohydrate transport and metabolism
A4Y4N4	707	0.66		Sputcn32_1189	Aldehyde ferredoxin oxidoreductase, EC 1.2.7.5
A4Y7R1	131	0.67		Sputcn32_2273	Succinate dehydrogenase subunit C, EC 1.3.5.1
A4Y9V1	594	0.80	opgD	Sputcn32_3021	Glucans biosynthesis protein D
A4Y493	545	1.32	pgi	Sputcn32_1048	Glucose-6-phosphate isomerase, EC 5.3.1.9
A4Y6B4	164	1.41		Sputcn32_1772	4-Hydroxy-4-methyl-2-oxoglutarate aldolase, EC 4.1.1.112
A4Y9A6	404	1.43	deoB	Sputcn32_2820	Phosphopentomutase, EC 5.4.2.7
A4Y566	305	1.49		Sputcn32_1371	N-Acetylglucosamine kinase, EC 2.7.1.59
A4Y6K9	227	1.53		Sputcn32_1869	2-Keto-3-deoxy-phosphogluconate aldolase, EC 4.1.2.14
A4Y579	302	1.60		Sputcn32_1385	UTP--glucose-1-phosphate uridylyltransferase, EC 2.7.7.9
A4Y769	544	1.76	opgG	Sputcn32_2081	Glucans biosynthesis protein G

Cell cycle control, cell division, chromosome partitioning
A4Y3E0	370	0.73	zapE	Sputcn32_0743	Cell division protein ZapE

Cell wall/membrane/envelope biogenesis
A4Y6J0	192	0.59	rnfA	Sputcn32_1850	Ion-translocating oxidoreductase complex subunit A, EC 7.-.-.-
A4YBG8	460	0.59		Sputcn32_3593	Membrane fusion protein (MFP) family protein
A4Y5V0	178	0.61	pal	Sputcn32_1608	Peptidoglycan-associated protein
A4Y2N5	403	0.69	ftsW	Sputcn32_0486	Probable peptidoglycan glycosyltransferase FtsW, EC 2.4.1.129
A4YB04	244	0.70	zapD	Sputcn32_3427	Cell division protein ZapD
A4Y378	330	0.71		Sputcn32_0681	Mg2+ transporter protein, CorA family protein
A4Y3B5	186	0.75	lptC	Sputcn32_0718	Lipopolysaccharide export system protein LptC
A4Y8T7	395	0.77	bamB	Sputcn32_2649	Outer-membrane protein assembly factor BamB
A4Y3B6	183	1.72		Sputcn32_0719	3-Deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC, EC 3.1.3.45
A4YBR9	197	1.78	gmhA	Sputcn32_3694	Phosphoheptose isomerase, EC 5.3.1.28

Energy production and conversion
A4Y920	320	0.58	thrB	Sputcn32_2734	Homoserine kinase, EC 2.7.1.39
A4Y6S9	334	0.62	ruvB	Sputcn32_1939	Holliday junction ATP-dependent DNA helicase RuvB, EC 3.6.4.12
A4Y398	545	0.68	groL groEL	Sputcn32_0701	60 kDa chaperonin
A4Y7T7	637	0.68	htpG	Sputcn32_2299	Chaperone protein HtpG
A4YCH7	142	0.68	atpC	Sputcn32_3955	ATP synthase epsilon chain (ATP synthase F1 sector epsilon subunit) (F-ATPase epsilon subunit)
A4YCH9	286	0.69	atpG	Sputcn32_3957	ATP synthase gamma chain
A4Y6J2	668	0.69	uvrB	Sputcn32_1852	UvrABC system protein B, protein UvrB
A4Y3P6	410	0.69	nqrB	Sputcn32_0850	Na (+)-translocating NADH-quinone reductase subunit B, Na (+)-NQR subunit B, Na (+)-translocating NQR subunit B, EC 7.2.1.1
A4Y2C0	470	0.70	ntrC	Sputcn32_0371	DNA-binding transcriptional regulator NtrC
A4Y4K2	596	0.70	lepA	Sputcn32_1157	Elongation factor 4, EF-4, EC 3.6.5.n1
A4YBH4	670	0.73	rep	Sputcn32_3599	ATP-dependent DNA helicase Rep, EC 3.6.4.12
A4YAH6	604	0.73	dsbD	Sputcn32_3247	Thiol:disulfide interchange protein DsbD, EC 1.8.1.8
A4Y3F5	468	0.74		Sputcn32_0758	Replicative DNA helicase, EC 3.6.4.12
A4YCH8	463	0.75	atpD	Sputcn32_3956	ATP synthase subunit beta, EC 7.1.2.2
A4YAV2	722	0.75		Sputcn32_3373	DNA helicase, EC 3.6.4.12
A4Y1A4	461	0.76	dnaA	Sputcn32_0001	Chromosomal replication initiator protein DnaA
A4Y934	856	0.76	mutS	Sputcn32_2748	DNA mismatch repair protein MutS
A4YCI0	513	0.76	atpA	Sputcn32_3958	ATP synthase subunit alpha, EC 7.1.2.2
A4Y4B7	429	0.76		Sputcn32_1072	NADH dehydrogenase, EC 1.6.99.3
A4Y675	720	0.77	ppk	Sputcn32_1733	Polyphosphate kinase, EC 2.7.4.1
A4Y965	149	0.77	nrdR	Sputcn32_2779	Transcriptional repressor NrdR
A4YBZ9	319	0.77	birA	Sputcn32_3775	Bifunctional ligase/repressor BirA
A4Y406	274	0.78	hmuV	Sputcn32_0960	Hemin import ATP-binding protein HmuV, EC 7.6.2.-
A4Y9Z6	494	0.78		Sputcn32_3066	NAD(P) transhydrogenase subunit beta, EC 7.1.1.1
A4Y3M4	464	0.79	mpl	Sputcn32_0827	UDP-N-acetylmuramate--L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptandioate ligase, EC 6.3.2.45
A4Y9A0	454	0.79	radA	Sputcn32_2814	DNA repair protein RadA
A4Y2J9	696	0.80	recG	Sputcn32_0450	ATP-dependent DNA helicase RecG, EC 3.6.4.12
A4Y470	857	0.80	clpB	Sputcn32_1024	Chaperone protein ClpB
A4Y8E4	192	0.80	tdk	Sputcn32_2506	Thymidine kinase, EC 2.7.1.21
A4Y9N9	207	0.81		Sputcn32_2957	Corrinoid adenosyltransferase, EC 2.5.1.17
A4Y5P9	602	0.81	msbA	Sputcn32_1557	Lipid A export ATP-binding/permease protein MsbA, EC 7.5.2.6
A4Y427	388	0.82	obg	Sputcn32_0981	GTPase Obg, EC 3.6.5.-
A4Y4E0	378	0.83	potA	Sputcn32_1095	Spermidine/putrescine import ATP-binding protein PotA, EC 7.6.2.11
A4Y8T3	525	1.20	guaA	Sputcn32_2645	GMP synthase (glutamine-hydrolyzing), EC 6.3.5.2
A4YBZ8	316	1.21	coaA	Sputcn32_3774	Pantothenate kinase, EC 2.7.1.33
A4Y8F6	571	1.22	proS	Sputcn32_2518	Proline--tRNA ligase, EC 6.1.1.15
A4Y9M1	1074	1.22	carB	Sputcn32_2939	Carbamoyl-phosphate synthase large chain, EC 6.3.5.5
A4Y5H4	556	1.24	glnS	Sputcn32_1482	Glutamine--tRNA ligase, EC 6.1.1.18
A4Y334	640	1.25	deaD	Sputcn32_0637	ATP-dependent RNA helicase DeaD, EC 3.6.4.13
A4YBS3	332	1.25	trpS	Sputcn32_3698	Tryptophan--tRNA ligase, EC 6.1.1.2
A4Y7N1	789	1.25		Sputcn32_2243	Phosphoenolpyruvate synthase, EC 2.7.9.2
A4Y9M2	386	1.29	carA	Sputcn32_2940	Carbamoyl-phosphate synthase small chain, EC 6.3.5.5
A4Y5T8	345	1.32	purM	Sputcn32_1596	Phosphoribosylformylglycinamidine cyclo-ligase, EC 6.3.3.1
A4Y6M8	360	1.33	nadA	Sputcn32_1888	Quinolinate synthase A, EC 2.5.1.72
A4Y545	245	1.34	pyrH	Sputcn32_1350	Uridylate kinase, EC 2.7.4.22
A4Y2N4	439	1.37	murD	Sputcn32_0485	UDP-N-acetylmuramoylalanine--D-glutamate ligase, EC 6.3.2.9
A4Y4H3	397	1.37	tyrS	Sputcn32_1128	Tyrosine--tRNA ligase, EC 6.1.1.1
A4YA38	550	1.37	rhlE	Sputcn32_3108	ATP-dependent RNA helicase RhlE, EC 3.6.4.13
A4Y928	958	1.38	valS	Sputcn32_2742	Valine--tRNA ligase, EC 6.1.1.9
A4Y9Z3	565	1.40	cysI	Sputcn32_3063	Sulfite reductase [NADPH] hemoprotein beta-component, EC 1.8.1.2
A4Y959	323	1.41	thiL	Sputcn32_2773	Thiamine-monophosphate kinase, EC 2.7.4.16
A4Y6G8	327	1.42	pheS	Sputcn32_1828	Phenylalanine--tRNA ligase alpha subunit, EC 6.1.1.20
A4Y7E3	231	1.43	lolD	Sputcn32_2155	Lipoprotein-releasing system ATP-binding protein LolD, EC 7.6.2.-
A4YBL9	292	1.44	prpB	Sputcn32_3644	2-Methylisocitrate lyase, 2-MIC, EC 4.1.3.30
A4Y4A4	940	1.46	ileS	Sputcn32_1059	Isoleucine--tRNA ligase, EC 6.1.1.5
A4Y5I7	401	1.49	fabV	Sputcn32_1495	Enoyl-[acyl-carrier-protein] reductase [NADH], EC 1.3.1.9
A4YA91	317	1.51	gshB	Sputcn32_3162	Glutathione synthetase, EC 6.3.2.3
A4Y7C9	143	1.53	ndk	Sputcn32_2141	Nucleoside diphosphate kinase, EC 2.7.4.6
A4Y6Z5	428	1.55	serS	Sputcn32_2007	Serine--tRNA ligase, EC 6.1.1.11
A4Y9Q3	639	1.60	dnaK	Sputcn32_2971	Chaperone protein DnaK
A4Y2H2	198	1.71	azoR	Sputcn32_0423	FMN-dependent NADH-azoreductase, EC 1.7.1.17
A4YAY0	432	2.11	purD	Sputcn32_3402	Phosphoribosylamine--glycine ligase, EC 6.3.4.13
A4Y3S0	391	2.25	pgk	Sputcn32_0874	Phosphoglycerate kinase, EC 2.7.2.3
A4Y397	96	3.88	groS groES	Sputcn32_0700	10 kDa chaperonin

Lipid transport and metabolism
A4Y4F4	203	0.54	plsY	Sputcn32_1109	G3P acyltransferase, GPAT, EC 2.3.1.275
A4Y9G1	219	0.70	lipB	Sputcn32_2875	Octanoyltransferase, EC 2.3.1.181
A4Y625	238	0.72	fadR	Sputcn32_1683	Fatty acid metabolism regulator protein
A4Y898	436	0.72	fadI	Sputcn32_2460	3-Ketoacyl-CoA thiolase, EC 2.3.1.16
A4Y553	341	0.75	lpxD	Sputcn32_1358	UDP-3-O-acylglucosamine N-acyltransferase, EC 2.3.1.-
A4Y5S4	248	1.22		Sputcn32_1582	3-Oxoacyl-[acyl-carrier-protein] reductase, EC 1.1.1.100
A4Y4D0	498	1.34		Sputcn32_1085	Aldehyde dehydrogenase (acceptor), EC 1.2.5.2
A4Y7Z3	354	1.38	fabH	Sputcn32_2355	3-Oxoacyl-[acyl-carrier-protein] synthase 3, EC 2.3.1.180
A4Y3G1	245	1.49		Sputcn32_0764	Enoyl-CoA hydratase, EC 4.2.1.17
A4Y5S3	308	1.74		Sputcn32_1581	Malonyl CoA-acyl carrier protein transacylase, EC 2.3.1.39
A4Y883	411	1.83		Sputcn32_2445	3-Oxoacyl-[acyl-carrier-protein] synthase I, EC 2.3.1.41
A4Y5X9	171	2.05	fabA	Sputcn32_1637	3-Hydroxydecanoyl-[acyl-carrier-protein] dehydratase, EC 4.2.1.59
A4Y5S5	77	2.54	acpP	Sputcn32_1583	Acyl carrier protein, ACP

Nucleotide transport and metabolism
A4Y951	735	0.64		Sputcn32_2765	(P)ppGpp synthetase I, SpoT/RelA, EC 2.7.6.5
A4Y9S5	343	1.32	pyrC	Sputcn32_2994	Dihydroorotase, EC 3.5.2.3
A4Y5T9	208	1.33	upp	Sputcn32_1597	Uracil phosphoribosyltransferase, EC 2.4.2.9
A4Y7I2	339	1.37	pyrD	Sputcn32_2194	Dihydroorotate dehydrogenase, EC 1.3.5.2
A4YAX9	543	1.46	purH	Sputcn32_3401	Bifunctional purine biosynthesis protein PurH (includes phosphoribosylaminoimidazolecarboxamide formyltransferase, EC 2.1.2.3 (AICAR transformylase); IMP cyclohydrolase, EC 3.5.4.10 (ATIC))
A4Y8X8	205	1.49		Sputcn32_2692	dITP/XTP pyrophosphatase, EC 3.6.1.66
A4Y9A7	443	1.54	deoA	Sputcn32_2821	Thymidine phosphorylase, EC 2.4.2.4
A4Y2U2	252	1.55		Sputcn32_0543	Uridine phosphorylase, EC 2.4.2.3
A4YCD7	331	1.62	add	Sputcn32_3914	Adenosine deaminase, EC 3.5.4.4
A4Y2C8	103	1.64	ppnP	Sputcn32_0379	Pyrimidine/purine nucleoside phosphorylase, EC 2.4.2.2
A4Y5R0	296	1.71	cdd	Sputcn32_1568	Cytidine deaminase, EC 3.5.4.5
A4Y7B5	245	1.80	kdsB	Sputcn32_2127	8-Amino-3,8-dideoxy-manno-octulosonate cytidylyltransferase, EC 2.7.7.90
A4Y7K5	193	1.99	dcd	Sputcn32_2217	dCTP deaminase, EC 3.5.4.13
A4Y726	231	2.08	pyrF	Sputcn32_2038	Orotidine 5′-phosphate decarboxylase, EC 4.1.1.23
A4Y4P9	145	2.97		Sputcn32_1204	Cytosine deaminase, EC 3.5.4.1

Replication, recombination, and repair
A4Y6I4	213	0.74	nth	Sputcn32_1844	Endonuclease III, EC 4.2.99.18
A4Y5Y2	609	0.82	uvrC	Sputcn32_1640	UvrABC system protein C, protein UvrC
A4Y9F1	343	0.82		Sputcn32_2865	DNA polymerase III, delta subunit, EC 2.7.7.7
A4Y6M1	185	1.24	seqA	Sputcn32_1881	Negative modulator of initiation of replication
A4Y8J7	237	1.24	fliA	Sputcn32_2559	RNA polymerase sigma factor FliA
A4Y729	95	1.43	ihfB himD	Sputcn32_2041	Integration host factor subunit beta, IHF-beta
A4YBK6	92	1.63	rpoZ	Sputcn32_3631	DNA-directed RNA polymerase subunit omega, EC 2.7.7.6
A4Y9D3	158	1.65	greA	Sputcn32_2847	Transcription elongation factor GreA
A4YB05	69	2.50	yacG	Sputcn32_3428	DNA gyrase inhibitor YacG

Secondary metabolites biosynthesis, transport, and catabolism
A4YAT1	493	0.62	ubiD	Sputcn32_3352	3-Octaprenyl-4-hydroxybenzoate carboxy-lyase, EC 4.1.1.98
A4Y760	222	0.64		Sputcn32_2072	HAD-superfamily hydrolase, subfamily IA, variant 1, EC 3.1.3.18
A4Y3M6	176	0.69		Sputcn32_0829	Hypoxanthine phosphoribosyltransferase, EC 2.4.2.8
A4Y930	418	0.71		Sputcn32_2744	Aspartokinase, EC 2.7.2.4
A4YAL8	289	0.73	psd	Sputcn32_3289	Phosphatidylserine decarboxylase proenzyme, EC 4.1.1.65 (cleaved into phosphatidylserine decarboxylase alpha chain; phosphatidylserine decarboxylase beta chain)
A4Y7Q6	400	0.74		Sputcn32_2268	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, EC 2.3.1.61
A4Y9A3	331	0.75		Sputcn32_2817	Phosphoserine phosphatase, EC 3.1.3.3
A4Y7C7	164	0.76		Sputcn32_2139	Acetolactate synthase, small subunit, EC 2.2.1.6
A4Y719	437	0.79		Sputcn32_2031	Phospholipase D/transphosphatidylase EC 2.7.8.8
A4Y9C6	277	0.82		Sputcn32_2840	Dihydropteroate synthase, EC 2.5.1.15
A4Y2M0	522	1.21	leuA	Sputcn32_0471	2-Isopropylmalate synthase, EC 2.3.3.13
A4Y9M3	270	1.22	dapB	Sputcn32_2941	4-Hydroxy-tetrahydrodipicolinate reductase, EC 1.17.1.8
A4Y9W8	173	1.27		Sputcn32_3038	Protoporphyrinogen oxidase, EC 1.3.3.4
A4Y2M2	474	1.29	leuC	Sputcn32_0473	3-Isopropylmalate dehydratase large subunit, EC 4.2.1.33
A4Y7E1	267	1.31		Sputcn32_2153	Inositol-1-monophosphatase, EC 3.1.3.25
A4Y4A7	318	1.32	ispH	Sputcn32_1062	4-Hydroxy-3-methylbut-2-enyl diphosphate reductase, EC 1.17.7.4
A4Y4T4	440	1.34		Sputcn32_1239	Isocitrate lyase, EC 4.1.3.1
A4Y3R8	664	1.35		Sputcn32_0872	Transketolase, EC 2.2.1.1
A4YAZ4	888	1.39		Sputcn32_3417	Pyruvate dehydrogenase E1 component, EC 1.2.4.1
A4YBI7	619	1.40	ilvD	Sputcn32_3612	Dihydroxy-acid dehydratase, EC 4.2.1.9
A4Y2M1	364	1.42	leuB	Sputcn32_0472	3-Isopropylmalate dehydrogenase, EC 1.1.1.85
A4Y486	163	1.44	purE	Sputcn32_1041	N5-carboxyaminoimidazole ribonucleotide mutase, N5-CAIR mutase, EC 5.4.99.18
A4Y6E8	514	1.48		Sputcn32_1807	Fumarate hydratase class I, EC 4.2.1.2
A4Y4X1	293	1.49		Sputcn32_1276	Farnesyl-diphosphate synthase, EC 2.5.1.10
A4Y5G2	394	1.49		Sputcn32_1470	Glycerate kinase, EC 2.7.1.31
A4Y424	331	1.51		Sputcn32_0978	Trans-hexaprenyltranstransferase, EC 2.5.1.30
A4Y7N3	357	1.54		Sputcn32_2245	Phospho-2-dehydro-3-deoxyheptonate aldolase, EC 2.5.1.54
A4Y591	396	1.55		Sputcn32_1397	Acetyl-CoA acetyltransferase, EC 2.3.1.9
A4YAY7	865	1.58		Sputcn32_3409	Aconitate hydratase B, EC 4.2.1.3
A4Y9W4	217	1.60		Sputcn32_3034	Dihydropteridine reductase, EC 1.5.1.34
A4Y670	288	1.63		Sputcn32_1728	Acyl-CoA thioesterase II, EC 3.1.2.2
A4YAE8	311	1.66	mdh	Sputcn32_3219	Malate dehydrogenase, EC 1.1.1.37
A4Y494	318	1.66	tal	Sputcn32_1049	Transaldolase, EC 2.2.1.2
A4YAY4	354	1.68	hemE	Sputcn32_3406	Uroporphyrinogen decarboxylase, EC 4.1.1.37
A4Y5N5	203	1.74	ribA	Sputcn32_1543	GTP cyclohydrolase-2, EC 3.5.4.25
A4Y1D3	302	1.75	hemF	Sputcn32_0030	Oxygen-dependent coproporphyrinogen-III oxidase, EC 1.3.3.3
A4YBS4	226	1.75	gph	Sputcn32_3699	Phosphoglycolate phosphatase, EC 3.1.3.18
A4Y4G4	430	1.77	hemL	Sputcn32_1119	Glutamate-1-semialdehyde 2,1-aminomutase, EC 5.4.3.8
A4Y644	294	1.96	dapA	Sputcn32_1702	4-Hydroxy-tetrahydrodipicolinate synthase, EC 4.3.3.7
A4Y846	278	2.00	trpA	Sputcn32_2408	Tryptophan synthase alpha chain, EC 4.2.1.20
A4YBG2	310	2.04	hemC	Sputcn32_3587	Porphobilinogen deaminase, EC 2.5.1.61
A4Y7T5	214	2.14	adk	Sputcn32_2297	Adenylate kinase, EC 2.7.4.3
A4Y1E3	514	2.14	gpmI	Sputcn32_0040	2,3-Bisphosphoglycerate-independent phosphoglycerate mutase, EC 5.4.2.12
A4Y881	338	2.14	asd	Sputcn32_2443	Aspartate-semialdehyde dehydrogenase, EC 1.2.1.11
A4Y9L3	220	2.15	rpiA	Sputcn32_2931	Ribose-5-phosphate isomerase A, EC 5.3.1.6
A4Y966	417	2.21	glyA	Sputcn32_2780	Serine hydroxymethyltransferase, SHMT, EC 2.1.2.1
A4Y3V1	597	2.22		Sputcn32_0905	Fumarate reductase flavoprotein subunit, EC 1.3.5.4
A4Y452	292	2.27		Sputcn32_1006	Cysteine synthase, EC 2.5.1.47
A4Y943	431	2.31	eno	Sputcn32_2757	Enolase, EC 4.2.1.11
A4Y7M6	344	2.41		Sputcn32_2238	Leucine dehydrogenase, EC 1.4.1.9

Transcription
A4Y9B8	318	0.63	truB	Sputcn32_2832	tRNA pseudouridine synthase B, EC 5.4.99.25
A4Y9L4	396	0.79	rlmI	Sputcn32_2932	Ribosomal RNA large subunit methyltransferase I, EC 2.1.1.191
A4Y9C1	499	1.27	nusA	Sputcn32_2835	Transcription termination/antitermination protein NusA
A4YB21	101	1.31	fis	Sputcn32_3444	DNA-binding protein Fis
A4Y2K5	237	1.31	rph	Sputcn32_0456	Ribonuclease PH, EC 2.7.7.56
A4Y960	134	1.66	nusB	Sputcn32_2774	Transcription antitermination protein NusB

Translation, ribosomal structure, and biogenesis
A4Y5U6	228	0.67	tolQ	Sputcn32_1604	Tol-Pal system protein TolQ
A4Y7L6	102	0.70	clpS	Sputcn32_2228	ATP-dependent Clp protease adapter protein ClpS
A4YBZ6	123	0.71	secE	Sputcn32_3772	Protein translocase subunit SecE
A4YA83	697	0.73	fusA	Sputcn32_3154	Elongation factor G, EF-G
A4Y1K1	281	0.73	rlmJ	Sputcn32_0098	Ribosomal RNA large subunit methyltransferase J, EC 2.1.1.266
A4YBY2	201	0.73	rplD	Sputcn32_3758	50S ribosomal protein L4
A4Y836	291	0.75		Sputcn32_2398	Pseudouridine synthase, EC 5.4.99.-
A4YCI2	156	0.76	atpF	Sputcn32_3960	ATP synthase subunit b
A4YBX7	230	0.78	rpsC	Sputcn32_3753	30S ribosomal protein S3
A4YCI4	273	0.78	atpB	Sputcn32_3962	ATP synthase subunit a
A4Y8B9	315	0.79	secF	Sputcn32_2481	Protein-export membrane protein SecF
A4Y8C1	110	0.79	yajC	Sputcn32_2483	Sec translocon accessory complex subunit YajC
A4Y4K5	338	0.80	era	Sputcn32_1160	GTPase Era
A4YBY4	103	1.26	rpsJ	Sputcn32_3760	30S ribosomal protein S10
A4Y4S7	174	1.31	bamE	Sputcn32_1232	Outer-membrane protein assembly factor BamE
A4YBV9	206	1.32	rpsD	Sputcn32_3735	30S ribosomal protein S4
A4Y3E7	246	1.33	rlmB	Sputcn32_0750	23S rRNA (guanosine-2′-O-)-methyltransferase RlmB, EC 2.1.1.185
A4YBW1	118	1.41	rpsM	Sputcn32_3737	30S ribosomal protein S13
A4Y3E1	142	1.42	rplM	Sputcn32_0744	50S ribosomal protein L13
A4Y9C2	151	1.42	rimP	Sputcn32_2836	Ribosome maturation factor RimP
A4YBW4	144	1.44	rplO	Sputcn32_3740	50S ribosomal protein L15
A4Y544	283	1.45	tsf	Sputcn32_1349	Elongation factor Ts, EF-Ts
A4YBW0	130	1.47	rpsK	Sputcn32_3736	30S ribosomal protein S11
A4Y3E2	130	1.49	rpsI	Sputcn32_0745	30S ribosomal protein S9
A4Y705	143	1.56	infC	Sputcn32_2017	Translation initiation factor IF-3
A4Y6A5	85	1.57	minE	Sputcn32_1763	Cell division topological specificity factor
A4Y3F3	150	1.61	rplI	Sputcn32_0756	50S ribosomal protein L9
A4YBX9	92	1.67	rpsS	Sputcn32_3755	30S ribosomal protein S19
A4YBY8	124	1.71	rpsL	Sputcn32_3764	30S ribosomal protein S12
A4YBV7	131	1.72	rplQ	Sputcn32_3733	50S ribosomal protein L17
A4YBW5	60	1.72	rpmD	Sputcn32_3741	50S ribosomal protein L30
A4Y5S0	56	1.75	rpmF	Sputcn32_1578	50S ribosomal protein L32
A4Y9F3	109	1.77	rsfS	Sputcn32_2867	Ribosomal silencing factor RsfS
A4Y9B7	89	1.84	rpsO	Sputcn32_2831	30S ribosomal protein S15
A4YBY7	156	1.86	rpsG	Sputcn32_3763	30S ribosomal protein S7
A4Y704	64	1.87	rpmI	Sputcn32_2016	50S ribosomal protein L35
A4Y7L4	72	1.90	infA	Sputcn32_2226	Translation initiation factor IF-1
A4Y426	84	1.90	rpmA	Sputcn32_0980	50S ribosomal protein L27
A4Y4L3	83	1.90	rpsP	Sputcn32_1168	30S ribosomal protein S16
A4YBM9	73	1.92	zapB	Sputcn32_3654	Cell division protein ZapB
A4YBX6	136	1.93	rplP	Sputcn32_3752	50S ribosomal protein L16
A4Y6L5	186	1.96	efp	Sputcn32_1875	Elongation factor P, EF-P
A4Y3F2	75	2.05	rpsR	Sputcn32_0755	30S ribosomal protein S18
A4Y4A1	88	2.09	rpsT	Sputcn32_1056	30S ribosomal protein S20
A4Y5V1	249	2.09	cpoB	Sputcn32_1609	Cell division coordinator CpoB
A4Y6Z8	208	2.14	lolA	Sputcn32_2010	Outer-membrane lipoprotein carrier protein
A4YB74	154	2.17	trmL	Sputcn32_3498	tRNA (cytidine (34)-2′-O)-methyltransferase, EC 2.1.1.207
A4YBX8	110	2.21	rplV	Sputcn32_3754	50S ribosomal protein L22
A4YBX2	104	2.22	rplX	Sputcn32_3748	50S ribosomal protein L24
A4Y2V2	79	2.28	rpmE	Sputcn32_0553	50S ribosomal protein L31
A4Y546	185	2.28	frr	Sputcn32_1351	Ribosome-recycling factor, RRF
A4YBW7	116	2.44	rplR	Sputcn32_3743	50S ribosomal protein L18
A4YBX0	101	2.61	rpsN	Sputcn32_3746	30S ribosomal protein S14
A4Y703	118	2.62	rplT	Sputcn32_2015	50S ribosomal protein L20
A4Y2L3	57	3.30	rpmG	Sputcn32_0464	50S ribosomal protein L33
A4Y5X8	58	3.61	rmf	Sputcn32_1636	Ribosome modulation factor, RMF
A4YCM5	45	3.83	rpmH	Sputcn32_4005	50S ribosomal protein L34
A4YBW2	37	4.44	rpmJ	Sputcn32_3738	50S ribosomal protein L36

Not assigned
A4Y655	218	1.41		Sputcn32_1713	Butyryl-CoA:acetate CoA transferase, EC 2.8.3.8

¹UniProt and NCBI database accession numbers. ²Average ratio from three replicates by TMT experiment. A protein species was considered differentially accumulated if it exhibited a fold change >1.5-fold (cultivated at 4°C/cultivated at 30°C) with a value of <0.05.