Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii

Table 1

¹³C-NMR (CD₃OD, 200 MHz) data of compounds 1 and 2.

C	1	2	C	1	2
1	35.7	35.7	17	58.1	58.6
2	71.4	71.4	18	19.3	19.3
3	212.5	212.5	19	18.3	18.7
4	50.4	50.3	20	78.5	78.8
5	140.6	140.7	21	24.0	23.9
6	119.8	119.8	22	203.5	203.8
7	23.4	23.4	23	119.8	121.2
8	42.7	42.7	24	153.9	150.1
9	47.9	48.2	25	70.2	79.6
10	33.4	33.4	26	28.4	25.5
11	214.2	214.4	27	27.8	25.0
12	48.4	48.4	28	20.4	20.4
13	47.9	48.0	29	27.7	28.4
14	50.4	50.4	30	18.1	18.0
15	45.2	45.0	CH3CO		20.4
16	70.1	70.5	CH3CO		170.5

Shift values (δ) are expressed in ppm.