Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii

Table 2

Solvent gradients for HPLC analysis.

Time (min)	% ACN	% DW
Initial	15	85
10	60	40
20	80	20
30	100	0
35	15	85