Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr class="thead"><td class="align_left" rowspan="2">Sample</td><td class="align_center" rowspan="2">Retention time (min)</td><td class="align_center" rowspan="2">Linear regression equation</td><td class="align_center" rowspan="2">Correlation coefficient (R2)</td><td class="align_center">LOD</td><td class="align_center">LOQ</td></tr><tr class="thead"><td class="align_center" colspan="2">(μg/mL)</td></tr><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr><td class="align_left">Cucurbitacin B</td><td class="align_center">15.450 ± 0.042</td><td class="align_center">y = 2960.9x + 2153.7</td><td class="align_center">0.999</td><td class="align_center">1.87</td><td class="align_center">5.66</td></tr><tr><td class="align_left">Cucurbitacin D</td><td class="align_center">12.589 ± 0.046</td><td class="align_center">y = 2621.2x + 1950.8</td><td class="align_center">1</td><td class="align_center">1.30</td><td class="align_center">3.93</td></tr><tr class="table-tr"><td colspan="6"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Analytical figure of merit of the proposed method for the determination of cucurbitacins B and D.</div>

Journal of Chemistry

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Table 3

Table 3: Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii

Table 3 | Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii 