Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii

Table 4

Analytical results of intra- and inter-day precision of cucurbitacins B and D.

Sample	Conc. (µg/mL)	Intra-day		Inter-day
		Observed conc. (µg/mL)	RSD (%)	Observed conc. (µg/mL)	RSD (%)
Cucurbitacin B	50	50.3 ± 0.60	1.19	51.7 ± 0.38	0.74
	100	103.4 ± 0.85	0.82	103.2 ± 1.13	1.09
	250	248.9 ± 3.14	1.26	249.0 ± 3.27	1.31
Cucurbitacin D	50	50.9 ± 0.50	0.98	51.0 ± 0.69	1.35
	100	100.8 ± 0.34	0.34	100.1 ± 0.61	0.61
	250	249.7 ± 1.90	0.76	247.9 ± 0.64	0.26

Intra- and inter-day: three times per day and two times analysis of cucurbitacins B and D for three days, respectively.