Development and Validation of a New Analytical HPLC-PDA Method for Simultaneous Determination of Cucurbitacins B and D from the Roots of Trichosanthes kirilowii

Table 5

Recovery results for cucurbitacins B and D (n = 3).

Compound	Spiked amount (μg/mL)	Measured amount (μg/mL)	Recovery (%)^a	RSD (%)
Cucurbitacin B	25.0	25.4 ± 0.15	101.7 ± 0.61	0.60
	50.0	49.6 ± 0.65	99.2 ± 1.30	1.31
	75.0	75.7 ± 0.08	101.0 ± 0.11	0.11
Cucurbitacin D	25.0	25.5 ± 0.10	102.0 ± 0.41	0.40
	50.0	49.3 ± 0.74	98.6 ± 1.47	1.49
	75.0	75.6 ± 0.61	100.8 ± 0.82	0.81

^aRecovery (%) = (found amount−original amount)/spiked amount ×100.