Improving the Extraction of Active Ingredients from Medicinal Plants by XynA Modification

Figure 3

Enzyme engineering and protein expression. (a) Schematic domain of XynA, with boundaries indicated. The N-terminal domain, GH10 domain, and C-terminal domain are coloured purple, cyan, and pale green, respectively. Below are some truncations designed to improve the enzyme activity: XynA_ΔC (39.28 kDa) represents the removal of the C-terminal domain; XynA_ΔN (40.70 kDa) represents the removal of the N-terminal domain; XynA_ΔN1-36 (44.32 kDa) represents the removal of residues 1–36 at the N-terminal domain; and XynA_ΔNC57-349 (35.40 kDa) represents the removal of residues 1–56 at the N-terminus and residues 350–408 at the C-terminal domain. (b)–(e) 12% SDS-PAGE analyses of XynA’s expression. M, marker; UI, uninitiated; I, initiation; SUP, supernatant; PPT, precipitation; UB, unbound protein; W, wash protein; and E, elution.

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