Improving the Extraction of Active Ingredients from Medicinal Plants by XynA Modification

<div>Biochemical analysis of XynA_ΔN36 and comparison of natural medicinal herb extraction for wild type and truncation. (a) Chromatogram of XynA_ΔN36 purified using a Hitrap QHP (1 mL) column. UV absorptions at 254 nm and 280 nm are coloured red and blue, respectively. In addition, the peak for XynA_ΔN36 is labelled. The black line represents ionic strength. (b) 12% SDS-PAGE analysis of purified XynA_ΔN36 protein. (c) Chromatogram of XynA_ΔN36 and XynA analysed using Superdex 200 increase 10/300 GL. XynA_ΔN36 and XynA are coloured blue and black, respectively. (d) Comparison of XynA and XynA_ΔN36 for salvianic acid A extraction. (e) Comparison of XynA and XynA_ΔN36 for berberine. XynA and XynA_ΔN36 are coloured green and pink, respectively. The <i>Y</i>-axis shows the berberine extraction efficiency (mg/g) with different enzymes. The data represent the mean ± S.D. 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Journal of Chemistry

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Figure 4

Figure 4: Improving the Extraction of Active Ingredients from Medicinal Plants by XynA Modification 