Research Article
Evaluation of Multicomponent Changes of Schisandra chinensis Fruits with Different Drying Process by UPLC-QQQ-MS-Based Targeted Metabolomics Analysis
Table 2
Validation of quantitative UPLC-QQQ-MS of 10 lignans, 5 organic acids, and 9 oligosaccharide references in Schisandra chinensis.
| | Compounds | Ion pairs | Regression equation | | Linear range (μg/mL) | Limit of detection (ng/mL) | Limit of quantitation (ng/mL) | Precision
RSD(%) | Repeatability
RSD(%) | Recovery
(%) |
| | Schisandrol A | 433.16/415.14 | | 0.9965 | 5-20 | 0.72 | 2.63 | 1.35 | 1.72 | 99.97 | | Gomisin J | 389.11/358.15 | | 0.9968 | 1-15 | 0.98 | 3.34 | 1.32 | 2.07 | 98.64 | | Schisandrol B | 417.12/399.12 | | 0.9978 | 1-10 | 1.26 | 4.07 | 2.42 | 2.25 | 99.08 | | Schisantherin A | 537.13/415.10 | | 0.9959 | 0.1-5 | 1.03 | 3.35 | 1.29 | 1.96 | 99.78 | | Schisanhenol | 403.14/372.11 | | 0.9970 | 0.1-3 | 1.79 | 5.83 | 2.17 | 3.25 | 97.72 | | Schizandrin A | 417.17/386.14 | | 0.9970 | 2-10 | 1.94 | 6.57 | 0.96 | 1.59 | 98.37 | | Schizandrin B | 401.12/371.09 | | 0.9978 | 1-20 | 1.53 | 5.09 | 0.83 | 1.14 | 99.53 | | Schisandrin C | 385.10/355.08 | | 0.9986 | 1-10 | 0.67 | 2.36 | 1.32 | 1.75 | 98.42 | | Gomisin D | 531.30/500.67 | | 0.9993 | 0.1-4 | 0.85 | 2.85 | 1.27 | 1.58 | 98.54 | | Schisantherin B | 515.29/415.14 | | 0.9982 | 0.1-3 | 2.19 | 6.87 | 1.03 | 1.56 | 99.92 | | Quinic acid | 191.17/147.38 | | 0.9977 | 20-100 | 1.24 | 3.97 | 1.32 | 1.84 | 101.21 | | Malic acid | 133.18/115.20 | | 0.9973 | 150-550 | 1.46 | 4.71 | 1.75 | 1.92 | 101.47 | | Citric acid | 191.07/173.38 | | 0.9967 | 20-110 | 1.97 | 6.13 | 0.96 | 1.38 | 99.57 | | Sorbic acid | 134.45/111.19 | | 0.9994 | 20-110 | 0.89 | 2.96 | 1.05 | 1.61 | 101.28 | | Chlorogenic acid | 353.13/316.84 | | 0.9993 | 100-600 | 1.59 | 5.15 | 1.81 | 2.58 | 99.74 | | Maltose | 477.23/259.69 | | 0.9997 | 0.1-10 | 0.74 | 2.43 | 2.01 | 2.27 | 99.64 | | Maltotriose | 681.32/259.23 | | 0.9989 | 0.01-5 | 0.28 | 0.97 | 1.53 | 2.64 | 98.37 | | Maltotetraose | 885.41/463.47 | | 0.9990 | 0.1-5 | 0.19 | 0.72 | 2.27 | 2.11 | 101.29 | | Maltopentaose | 1089.48/667.30 | | 0.9971 | 0.1-10 | 0.96 | 3.08 | 2.07 | 2.51 | 99.37 | | Maltohexaose | 1293.55/871.38 | | 0.9966 | 0.2-5 | 0.82 | 2.63 | 2.51 | 2.13 | 101.05 | | Maltoheptaose | 1497.63/1075.46 | | 0.9952 | 0.2-10 | 0.53 | 1.72 | 1.39 | 2.25 | 100.64 | | Sucrose | 477.23/259.65 | | 0.9965 | 1-15 | 0.78 | 2.57 | 1.63 | 2.58 | 99.72 | | Kestose | 681.32/259.39 | | 0.9950 | 0.001-5 | 0.21 | 0.73 | 2.21 | 2.54 | 101.72 | | Nystose | 885.41/463.01 | | 0.9970 | 0.01-5 | 0.49 | 1.76 | 2.15 | 2.38 | 98.79 |
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