Optimization of GC-MS Method for Analysis of Basil Essential Oils from Algeria: Design of Experiments and Exploratory Pattern Recognition

Table 3

Experimental design and method parameters used for the first two studies.

		First study	Second study
Experimental design		2^3 factorial design	Doehlert design
Factors/responses		3/2	3/4
Number of runs		11	18
Method parameters	Initial temperature	45°C	45°C
	Isotherm duration	5 min	5 and 10 min
	Slope	2 and 6°C/min	1 and 2°C/min
	Final temperature	180°C	80 and 180°C
	Isothermal step at FT	10 minutes	10 minutes
	Split ratio	and
	Gas flow rate	0.6 and 1.3 mL/min	1.3 mL/min

Method parameters in bold were varied during respective stages, and the rest were kept constant.