Simultaneous Determination of Six Components in Beagle Dog Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study of Three Different Yuanhu Zhitong Preparations

Table 5

Accuracy and precision of the six analytes in dog plasma (mean, n = 3 days, 6 replicates per day).

Analyte	Nominal concentration (ng/mL)	Intraday			Interday
		Measured concentration (ng/mL)	Precision (%RSD)	Accuracy (%bias)	Measured concentration (ng/mL)	Precision (%RSD)	Accuracy (%bias)
Cde	0.2	0.20	0.00	0.00	0.20	0.00	0.00
	4	4.20	3.30	5.00	4.00	6.30	0.00
	32	31.80	3.50	−0.62	31.40	5.50	−1.88
Thp	0.1	0.10	0.00	0.00	0.10	0.00	0.00
	2	2.20	1.80	10.00	2.20	2.70	10.00
	16	14.30	2.70	−10.63	14.90	5.80	-6.88
Thb	0.1	0.10	0.00	0.00	0.10	0.00	0.00
	2	2.00	4.50	0.00	2.00	6.30	0.00
	16	16.00	1.80	0.00	15.70	4.50	−1.88
Bcin	1	1.00	10.00	0.00	1.00	9.20	0.00
	20	21.10	3.40	5.50	21.20	3.80	6.00
	160	139.00	1.90	−13.13	143.70	5.30	−10.19
Thc	0.1	0.10	0.00	0.00	0.10	0.00	0.00
	2	2.10	3.60	5.00	2.10	5.50	5.00
	16	15.80	1.90	−1.25	15.80	3.70	−1.25
Bcol	0.2	0.20	0.00	0.00	0.20	0.00	0.00
	4	4.20	3.20	5.00	4.20	4.60	5.00
	32	28.30	2.30	−11.56	29.20	4.50	−8.75