Simultaneous Determination of Six Components in Beagle Dog Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study of Three Different Yuanhu Zhitong Preparations

Table 6

Extraction recovery and matrix effect of the six analytes and internal standard in dog plasma (mean, n = 6).

Analyte	Spiked concentration (ng/mL)	Extraction recovery		Matrix effect
		Mean	RSD%	Mean	RSD%
Cde	0.2	107.32	12.50	104.47	16.90
	4	109.38	4.64	107.92	4.61
	32	105.69	1.39	103.38	2.56
Thp	0.1	99.50	11.06	105.68	8.47
	2	114.02	3.57	111.99	4.50
	16	106.07	2.35	107.07	2.36
Thb	0.1	109.11	16.12	84.11	13.05
	2	111.57	2.80	85.50	6.10
	16	105.43	1.24	86.89	1.94
Bcin	1	119.52	6.53	118.44	9.96
	20	109.55	4.82	112.60	6.38
	160	109.19	2.95	106.22	4.91
Thc	0.1	108.91	9.58	87.03	19.15
	2	109.92	3.42	90.21	5.29
	16	107.02	2.40	85.43	2.49
Bcol	0.2	125.63	10.29	87.59	7.84
	4	120.47	5.06	82.85	5.08
	32	107.05	0.99	85.43	5.19
Is	10	125.50	6.43	112.06	2.75