Simultaneous Determination of Six Components in Beagle Dog Plasma by UPLC-MS/MS and Its Application to a Comparative Pharmacokinetic Study of Three Different Yuanhu Zhitong Preparations

Table 7

Stability of the six analytes in dog plasma under different conditions (mean, n = 6).

Analyte	Spiked concentration (ng/mL)	25°C for 4 h		4°C for 24 h		Three freeze-thaw cycles		−80°C frozen for 30 days
		Accuracy (%bias)	Precision (%RSD)	Accuracy (%bias)	Precision (%RSD)	Accuracy (%bias)	Precision (%RSD)	Accuracy (%bias)	Precision (%RSD)
Cde	0.2	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
	4	2.08	2.90	7.22	5.30	1.67	5.10	−7.92	3.20
	32	9.43	6.50	5.50	5.20	−4.06	2.80	−3.28	2.40
Thp	0.1	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
	2	−1.67	5.30	0.28	6.70	5.83	4.60	5.00	4.30
	16	4.06	9.30	1.08	5.10	−12.19	3.10	−9.17	3.50
Thb	0.1	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
	2	−4.17	7.70	−5.56	4.40	−2.50	5.40	−9.17	6.40
	16	3.33	9.30	−1.08	8.00	−0.63	1.40	−5.42	4.90
Bcin	1	8.33	9.10	1.11	7.50	5.00	5.20	−3.33	8.40
	20	−0.83	3.00	1.00	6.50	11.92	2.40	6.92	6.30
	160	0.91	8.70	−2.34	4.60	−7.22	2.70	−11.73	1.10
Thc	0.1	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
	2	0.83	7.30	−0.56	6.90	4.17	4.70	−7.50	6.60
	16	1.35	8.90	−4.62	4.50	−4.27	3.50	−7.81	3.10
Bcol	0.2	0.00	0.00	0.00	0.00	0.00	0.00	0.00	0.00
	4	5.00	2.10	6.25	3.70	8.75	2.40	6.67	5.70
	32	−1.15	7.60	−3.65	4.50	−9.53	2.50	−10.36	1.30