An Ultrafast Fluorescent Probe for the Detection of Peroxynitrite in Living Cells

Figure 1

Spectral characteristics of HND-ONOO. (a) UV-Vis absorption and (b) the fluorescence spectrum of probe toward ONOO⁻ (0–20 µM). (c) The linear relationship between the fluorescence intensity and ONOO⁻. (d) The response of HND-ONOO to various active substance: 1, blank; 2, ONOO⁻ (20 µM); 3, H₂O₂ (100 µM); 4, HClO (100 µM); 5, O₂•^- (100 µM); 6, •OH (100 µM); 7, ^tBuOO• (100 µM); 8, NO (100 µM); 9, HNO (100 µM); 10, GSH (10 mM); 11, Hcy (10 mM); 12, Cys (10 mM).

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