Ultrasound-Assisted Enzymatic Extraction and Bioactivity Analysis of Polypeptides from Cordyceps militaris

Table 4

Orthogonal optimization results of C. militaris protein extraction.

Number	pH	Material-to-water ratio (g/mL)	Ultrasonic extraction time (h)	Numbers of extraction (times)	Protein content (%)
1	1	1	1	1	37.02 ± 0.73
2	1	2	2	2	42.01 ± 0.85
3	1	3	3	3	42.02 ± 0.82
4	2	1	2	3	43.04 ± 0.56
5	2	2	3	1	43.01 ± 0.23
6	2	3	1	2	39.88 ± 0.22
7	2	2	3	2	44.62 ± 0.01
8	3	2	1	3	42.18 ± 0.41
9	3	3	2	1	44.31 ± 0.31
K1	121.23	32.31	26.72	33.18
K2	125.93	31.92	36.32	31.18
K3	131.11	32.51	39.42	32.19
k1	8.11	12.13	9.34	10.32
k2	11.22	12.03	12.16	11.98
k3	13.32	12.19	13.09	12.11
R	5.01	0.13	4.19	0.67
Order	A > C > D > B
Optima levels	A2	B2	C3	D2
Optimal combination	A2B2C3D2

Data represent means ± standard deviation (n = 5). Values in the same column with different superscripts are significantly different ().