Ultrasound-Assisted Enzymatic Extraction and Bioactivity Analysis of Polypeptides from Cordyceps militaris

Table 5

Results and analysis of the orthogonal test of C. militaris polypeptides.

Number	pH	Addition enzyme content (U/mL)	Temperature (°C)	Time (h)	Polypeptide content (%)
1	1	1	1	1	13.46 ± 0.05
2	1	2	2	2	16.42 ± 0.08
3	1	3	3	3	12.31 ± 0.08
4	2	1	2	3	15.46 ± 0.05
5	2	2	3	1	15.03 ± 0.12
6	2	3	1	2	14.98 ± 0.11
7	3	1	3	2	15.66 ± 0.32
8	3	2	1	3	16.12 ± 0.13
9	3	3	2	1	15.09 ± 0.09
K1	47.70	48.92	47.66	46.79
K2	47.78	49.04	48.77	48.76
K3	48.01	50.01	49.93	49.09
k1	16.13	15.66	16.23	15.77
k2	15.96	15.98	15.88	13.82
K3	15.99	14.98	14.97	16.01
R	0.26	2.01	2.13	1.09
Order	C > B > D > A
Optimal levels	A1	B2	C2	D2
Optimal combination	A1B2C2D2

Data represent means ± standard deviation (n = 5). Values in the same column with different superscripts are significantly different (P < 0.05).