Binding Investigation of Some Important Metal Ions Copper (I), Nickel (II), and Aluminium (III) with Bovine Serum Albumin Using Valid Spectroscopic Techniques

<div>Plot of 1/(A0 − A) vs. 1/Cmetal; A0 and A = the absorbance of BSA alone and BSA-metal complexes, respectively; Cmetal = concentration of Cu+, Ni+2, and Al+3. Buffer: tris (pH 7.4); CBSA = 12 µM; Cmetal = 0, 4, 8, 12, 16, 20, 24, 28, and 32 µM; (a) KCu+ = 3.46 × 104 M−1, (b) KNi2+ = 1.28 × 104 M−1, and (c) KAl3+ = 2.08 × 104 M−1 are the binding constants.</div>

Journal of Chemistry

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Figure 5

Figure 5: Binding Investigation of Some Important Metal Ions Copper (I), Nickel (II), and Aluminium (III) with Bovine Serum Albumin Using Valid Spectroscopic Techniques 

Figure 5 | Binding Investigation of Some Important Metal Ions Copper (I), Nickel (II), and Aluminium (III) with Bovine Serum Albumin Using Valid Spectroscopic Techniques 