Binding Investigation of Some Important Metal Ions Copper (I), Nickel (II), and Aluminium (III) with Bovine Serum Albumin Using Valid Spectroscopic Techniques

Table 2

Comparison of metal ions-protein interaction results from FT-IR and UV-Vis spectroscopy with those from ACE.

Metal ions	∆R/Rf ± cnf values in ACE [1]	% variation in α-helix after complexation	Binding constant (M^−1)
Cu^+	−0.0754 ± 0.0088	−29.03	3.46 × 10^4
Al^+3	−0.0484 ± 0.0087	−25.80	2.08 × 10^4
Ni^+2	−0.0397 ± 0.0037	−22.58	1.28 × 10^4

∆R: the mobility ratios of BSA in a capillary with and without metal ions vary. R_f: the mobility fraction of BSA in the lack of metal ions. Cnf: confidence interval calculated for six replicate tests.