Quality Evaluation of Bioactive Ingredients in Lianhua Qingwen Capsule Based on Quantitative Analysis of Multicomponent by the Single Marker Method and the Chemical Recognition Patterns Method

Table 5

Results of RCFs of different flow rates, column temperatures, and acidity of the mobile phase.

Influence factors		fNA/RHE	fAMY/RHE	fCA/RHE	fISF/RHE	fFA/RHE	fPIN/RHE	fQUE/RHE	fPHI/RHE	fGA/RHE
Flow rate (mL/min)	0.8	2.425	4.285	2.507	2.281	2.376	1.076	0.9005	0.7666	36.18
	1	2.496	4.389	2.609	2.195	2.541	1.015	0.9380	0.7952	35.19
	1.2	2.419	4.264	2.501	2.308	2.413	1.065	0.9133	0.7728	37.84
	Mean	2.446	4.313	2.539	2.261	2.443	1.052	0.9173	0.7782	36.41
	RSD (%)	1.75	1.56	2.40	2.61	3.55	3.09	2.08	1.93	3.67
Column temperature (°C)	29	2.604	4.591	2.669	2.041	2.572	0.8321	0.9817	0.8320	34.63
	30	2.639	4.649	2.701	2.195	2.623	0.8012	0.9920	0.8229	36.28
	31	2.541	4.456	2.601	2.220	2.509	0.8777	0.9578	0.8114	34.34
	Mean	2.595	4.565	2.657	2.152	2.568	0.8370	0.9772	0.8221	35.08
	RSD (%)	1.91	2.16	1.91	4.51	2.21	4.60	1.80	1.25	2.98
Phosphoric acid aqueous solution	0.05%	2.549	4.486	2.614	2.135	2.541	0.8808	0.9559	0.8161	34.79
	0.10%	2.496	4.389	2.609	1.975	2.523	0.8012	0.9380	0.7952	35.19
	0.15%	2.415	4.244	2.472	2.077	2.388	0.8285	0.9050	0.7736	36.94
	Mean	2.486	4.373	2.565	2.063	2.484	0.8369	0.9330	0.7950	35.64
	RSD (%)	2.70	2.78	3.15	3.92	3.37	4.83	2.77	2.67	3.21