Constituent Analysis and Quality Evaluation of Epimedii Folium from Different Species

Table 2

Optimized mass spectrometric parameters for MRM of 45 constituents.

No.	Constituents	Formula	tR(min)	Precursor ion (m/z)	Product ion (m/z)	DP (V)	CE (eV)	CXP (eV)	Ion mode
1	Lysine	C6H14N2O2	1.12	147.11	83.91	100	14	14	ESI^+
2	Histidine	C6H9N3O2	1.16	156.08	110.03	100	16	14	ESI^+
3	Serine	C3H7NO3	1.21	106.05	59.99	100	8	14	ESI^+
4	Glycine	C2H5NO2	1.21	76.04	30.00	73	6	14	ESI^+
5	Glutamic acid	C5H9NO4	1.22	148.10	83.90	12	14	14	ESI^+
6	Alanine	C3H7NO2	1.22	90.06	44.02	100	10	14	ESI^+
7	Threonine	C4H9NO3	1.22	120.17	74.00	100	20	14	ESI^+
8	Proline	C5H9NO2	1.31	116.07	70.02	68	10	14	ESI^+
9	Adenosine	C10H13N5O4	1.35	268.10	136.10	31	23	14	ESI^+
10	Uracil	C4H4N2O2	1.4	113.00	70.00	111	21	14	ESI^+
11	Valine	C5H11NO2	1.43	118.09	72.06	100	10	14	ESI^+
12	2′-deoxyadenosine	C10H13N5O3	1.72	251.81	136.08	80	9	6	ESI^+
13	Inosine	C10H12N4O5	2.04	269.00	137.07	46	15	14	ESI^+
14	Tyrosine	C9H11NO3	2.17	182.10	136.00	16	16	14	ESI^+
15	2′-deoxyinosine	C10H12N4O4	2.35	253.02	136.90	11	11	16	ESI^+
16	Leucine	C6H13NO2	2.9	132.10	86.05	100	16	14	ESI^+
17	Phenylalanine	C9H11NO2	4.99	166.10	120.05	100	14	14	ESI^+
18	Neochlorogenic acid	C16H18O9	5.76	353.02	190.96	−90	−24	−21	ESI^−
19	Chlorogenic acid	C16H18O9	7.83	353.05	191.03	−120	−22	−13	ESI^−
20	Cryptochlorogenic acid	C16H18O9	8.42	353.07	191.01	−105	−20	−21	ESI^−
21	Caffeic acid	C9H8O4	8.86	179.03	134.60	−125	−20	−15	ESI^−
22	Magnoflorine	C20H24NO4	9.83	343.10	298	125	24	14	ESI^+
23	Isoquercitrin	C21H20O12	13.15	463.00	300.00	−180	−36	−15	ESI^−
24	Hyperoside	C21H20O12	13.42	463.00	300.00	−160	−36	−15	ESI^−
25	Astragalin	C21H20O11	14.4	447.10	283.90	−100	−36	−15	ESI^−
26	Trifolin	C21H20O11	14.98	447.08	284.04	−195	−34	−19	ESI^−
27	Quercitrin	C21H20O11	14.99	447.00	301.00	−165	−30	−15	ESI^−
28	Afzelin	C21H20O10	16.75	431.10	285.00	−130	−40	−13	ESI^−
29	Epimedoside A	C32H38O15	16.98	663.234	355.219	121	35	26	ESI^+
30	Quercetin	C15H10O7	18.64	301.10	151.00	−62	−28	−15	ESI^−
31	Luteolin	C15H10O6	18.7	285.09	132.98	−170	−40	−15	ESI^−
32	Epimedin A1	C39H50O20	19.41	837.248	367.13	−185	−52	−45	ESI^−
33	Epimedin A	C39H50O20	19.8	837.238	675.249	−175	−20	−29	ESI^−
34	Epimedin B	C38H48O19	20.01	807.241	645.212	−170	−26	−31	ESI^−
35	Epimedin C	C39H50O19	20.33	821.261	659.271	−225	−14	−29	ESI^−
36	Sagittatoside A	C33H40O15	20.56	675.172	366.145	−180	−46	−27	ESI^−
37	Epimedin-I	C41H52O21	21.43	879.225	367.128	−195	−50	−31	ESI^-
38	Ikarisoside A	C26H28O10	23.61	499.089	353.141	−185	−36	−19	ESI^−
39	Icariside-I	C27H30O11	25.06	529.195	367.167	−230	−22	−17	ESI^−
40	Icaritin	C21H20O6	25.07	367.148	309.046	−240	−34	−37	ESI^−
41	Icariin	C33H40O15	25.17	677.21	369.219	181	37	28	ESI^+
42	Sagittatoside B	C32H38O14	25.51	645.254	366.162	−250	−44	−26	ESI^−
43	2′-O-Rhamnosyl icariside-II	C33H40O14	25.69	659.186	366.116	−300	−42	−33	ESI^−
44	Baohuoside-I	C27H30O10	26.06	513.114	366.108	−300	−36	−23	ESI^−
45	Anhydroicaritin	C21H20O6	29.31	367.149	352.107	−60	−28	−15	ESI^−