Constituent Analysis and Quality Evaluation of Epimedii Folium from Different Species

Table 3

Regression equations, limits of detection (LOD), limits of quantification (LOQ), precision, repeatability, stability, recovery, and matrix effect of 45 constituents.

No.	Constituents	Regression equation	r	Liner range (ng/mL)	LOD (ng/mL)	LOQ (ng/mL)	Precision (RSD, %)		Repeatability (RSD, %) (n = 6)	Stability (RSD, %) (n = 6)	Recovery (%)		Matrix effect
							Intraday (n = 6)	Interday (n = 9)			Mean	RSD
1	Lysine	Y = 1470X − 315000	0.9996	304–10100	2.563	8.542	1.19	2.37	4.78	1.24	97.78	0.99	0.94
2	Histidine	Y = 3850X − 37500	0.9994	32.4–5020	1.364	4.545	3.17	2.33	1.67	1.85	94.51	4.94	0.99
3	Serine	Y = 414X − 3220	0.9998	49.7–5950	3.371	11.238	3.89	3.77	2.97	2.38	97.09	2.96	0.96
4	Glycine	Y = 73.8X − 925	0.9993	96.8–11600	0.179	0.598	3.40	3.22	2.17	1.75	94.77	3.41	0.99
5	Glutamic acid	Y = 875X − 70300	0.9994	156–10600	2.371	7.903	1.80	4.52	3.09	2.61	102.17	0.66	0.94
6	Alanine	Y = 549X − 782	0.9999	281–10900	2.374	7.914	3.92	3.63	3.11	0.96	101.29	1.29	1.01
7	Threonine	Y = 481X − 14800	0.9998	104–11700	2.148	7.160	4.67	1.33	4.73	4.81	102.24	1.49	1
8	Proline	Y = 2320X − 18600	0.9995	323–12100	1.710	5.701	1.46	1.26	2.80	1.24	100.22	2.17	1.01
9	Adenosine	Y = 2610X − 26700	0.9993	442–12300	1.602	5.341	1.91	2.09	2.22	3.39	101.38	4.28	0.97
10	Uracil	Y = 376X − 5390	0.9999	31–8040	1.905	6.349	3.67	2.77	5.46	4.13	104.31	2.02	0.99
11	Valine	Y = 2010X − 260000	0.9993	435–14600	1.729	5.764	0.32	4.99	0.79	3.57	103.95	3.67	1.01
12	2′-deoxyadenosine	Y = 1060X − 790	0.9997	37.1–4280	0.365	1.216	0.39	2.59	2.71	1.10	97.37	3.69	1
13	Inosine	Y = 2360X − 728000	0.9993	514–13000	1.872	6.239	5	2.53	4.38	4.45	96.91	2.00	0.96
14	Tyrosine	Y = 3530X − 115000	0.9998	320–11000	0.725	2.417	4.12	2.33	3.35	3.48	97.29	2.52	1.02
15	2′-deoxyinosine	Y = 2560X − 251000	0.9998	361–13000	0.929	3.097	1.43	3.15	0.54	0.71	96.89	1.71	1.05
16	Leucine	Y = 2800X − 45900	0.9995	169–5530	0.738	2.460	2.79	2.86	1.43	1.71	97.54	1.92	0.98
17	Phenylalanine	Y = 3320X + 135000	0.9999	106–8410	1.033	3.442	1.59	1.99	5.44	0.82	99.14	0.62	0.96
18	Neochlorogenic acid	Y = 333X − 1850	0.9999	29.9–5300	8.792	29.306	2.33	1.56	3.34	4.76	102.21	4.13	0.99
19	Chlorogenic acid	Y = 3260X − 54.4	0.9995	35.5–6440	0.364	1.212	1.98	2.48	1.59	4.53	102.51	2.67	0.98
20	Cryptochlorogenic acid	Y = 1020X − 27200	0.9994	62.4–9140	1.517	5.055	1.52	4.80	3.50	3.20	95.20	2.81	0.98
21	Caffeic acid	Y = 4260X − 15100	0.9993	30.0–5260	3.757	12.524	1.09	3.36	1.72	3.22	97.87	2.40	0.95
22	Magnoflorine	Y = 895X + 2620000	0.9996	840–32400	0.387	1.291	1.77	3.73	1.95	3.87	101.27	1.38	1
23	Isoquercitrin	Y = 6750X − 44.2	0.9997	23.8–5400	0.293	0.977	1.26	3.30	2.84	1.35	95.33	3.59	1.02
24	Hyperoside	Y = 9890X − 15100	0.9999	23.6–2340	0.117	0.390	1.43	3.42	2.72	1.49	98.86	3.40	0.94
25	Astragalin	Y = 909X − 30600	0.9997	78.4–5040	0.534	1.780	2.95	3.29	3.51	2.31	105.66	2.14	0.97
26	Trifolin	Y = 3060X − 57400	0.9999	45.1–3450	0.105	0.351	0.97	1.41	1.97	2.75	99.45	0.64	0.97
27	Quercitrin	Y = 4610X − 17400	0.9996	32.1–5170	0.111	0.369	1.10	4.27	2.07	4.08	103.14	1.91	0.99
28	Afzelin	Y = 2050X − 138000	0.9995	173–13800	0.460	1.533	1.07	1.46	3.11	0.92	103.42	1.97	0.95
29	Epimedoside A	Y = 1880X − 132	0.9993	6.08–10400	0.274	0.912	3.56	1.96	3.06	4.03	97.24	2.05	0.96
30	Quercetin	Y = 4650X − 44700	0.9999	44.3–7760	0.351	1.170	1.12	3.28	4.94	4.06	104.31	3.52	0.99
31	Luteolin	Y = 8600X − 207000	0.9997	74.9–5680	0.085	0.283	1.76	2.91	4.84	2.43	96.65	2.06	0.97
32	Epimedin A1	Y = 1190X − 110000	0.9999	208–27300	0.404	1.347	0.87	3.84	2.04	3.99	99.07	4.72	1.01
33	Epimedin A	Y = 637X − 13500	0.9994	164–36200	0.524	1.745	0.47	1.89	1.77	2.42	97.89	4.87	1.02
34	Epimedin B	Y = 669X − 59000	0.9996	217–18500	0.363	1.210	0.55	2.25	1.71	2.21	103.85	3.32	1.04
35	Epimedin C	Y = 794X − 22900	0.9999	111–20600	0.464	1.546	1.37	0.62	1.08	1.49	104.77	3.92	0.99
36	Sagittatoside A	Y = 326X − 1250	0.9995	241–23800	0.194	0.647	1.92	0.71	2.31	1.14	98.80	3.11	0.92
37	Epimedin-I	Y = 666X − 1630	0.9994	7.14–31400	0.817	2.722	1.41	1.79	1.97	3.88	99.35	1.49	0.94
38	Ikarisoside A	Y = 3330X − 1590	0.9999	187–14700	0.161	0.535	1.08	1.68	1.55	2.86	97.39	4.43	1
39	Icariside-I	Y = 3820X − 3940	0.9998	17.6–8900	0.275	0.917	1.41	3.58	3.56	2.63	96.12	4.92	0.99
40	Icaritin	Y = 2500X − 51700	0.9997	207–10600	0.763	2.545	1.53	1.68	2.97	4.29	99.15	1.00	0.99
41	Icariin	Y = 1070X − 13200	0.9998	133–30100	0.144	0.479	3.96	4.58	2.17	3.44	104.42	1.42	1.04
42	Sagittatoside B	Y = 2820X − 2660	0.9993	46.8–11100	0.158	0.526	1.37	2.22	2.57	2.84	101.63	2.10	1.02
43	2′-O-Rhamnosyl icariside-II	Y = 1050X − 24000	0.9999	134–16300	0.180	0.601	2.85	4.86	4.85	0.43	101.00	3.81	1.04
44	Baohuoside-I	Y = 3080X − 316000	0.9998	141–7680	0.356	1.186	1.78	1.75	1.78	1.67	99.69	3.63	1.02
45	Anhydroicaritin	Y = 6590X − 267000	0.9996	97.6–10400	0.152	0.506	2.51	1.31	2.51	2.69	97.71	4.82	0.97