Development and Validation of an UHPLC-MS/MS Method for Quantification of DMAG in Rat Plasma and Its Application in a Preliminary Pharmacokinetic Study in Thrombocytopenia Rats

Table 4

Pharmacokinetic parameter of DMAG in rat after intragastric administration of normal and thrombocytopenia rats (n = 6).

Parameters	Normal rat		Thrombocytopenia rat
	2 mg/kg	4 mg/kg	2 mg/kg	4 mg/kg
AUC0–t (ng·h/mL)	114.58 ± 20.97	234.60 ±	148.05 ± 43.72	318.17 ± 24.49^▲▲##
AUC0–∞ (ng·h/mL)	146.58 ± 54.06	322.74 ±	237.45 ± 138.32	498.57 ± 52.66^▲▲##
MRT (h)	14.95 ± 9.25	18.15 ± 4.64	22.92 ± 13.94	26.32 ± 6.76^▲
t1/2 (h)	10.95 ± 7.35	15.34 ± 4.02	18.58 ± 11.53	24.84 ± 6.23
Tmax (h)	3.00 ± 0.00	3.00 ± 0.00	2.16 ±	2.33 ± 0.51^▲
Vz/F (mL/kg)	210756.33 ± 91244.18	274150.16 ± 50417.19	228507.00 ± 88363.40	286188.33 ± 50910.10
CL/F (mL/h/kg)	14799.66 ± 3889.18	12746.50 ± 2436.63	10303.66 ± 4027.34	8093.50 ± 806.26^▲▲
Cmax (ng/mL)	25.67 ± 2.68	49.13 ±	35.38 ±	67.78 ± 2.46^▲▲##

AUC_0–t, area under the concentration-time curve from 0 to 24 h; AUC_0–∞, area under the concentration-time curve from 0 to time infinite; MRT, mean residence time; t_1/2, half-life of elimination; T_max, time to achieve maximum concentration; V_z/F, volume of distribution; CL/F, clearance; C_max, maximum value of concentration; significant compared to normal rat treated with 2 mg/kg, ^▲, ^▲▲ compared to normal rat treated with 4 mg/kg, ^## compared to thrombocytopenia rat treated with 2 mg/kg.