Development and Validation of an UHPLC-MS/MS Method for Quantification of DMAG in Rat Plasma and Its Application in a Preliminary Pharmacokinetic Study in Thrombocytopenia Rats

Table 5

Pharmacokinetic parameter of DMAG in rat after intravenous administration of normal and thrombocytopenia rats with 2 mg/kg (n = 6).

Parameter	Normal rat	Thrombocytopenia rat
AUC0–t (ng·h/mL)	242.46 ± 27.01	589.02 ±
AUC0–∞ (ng·h/mL)	245.19 ± 27.48	605.58 ±
MRT (h)	1.79 ± 0.39	4.19 ± 2.30
t1/2 (h)	29.10 ± 8.36	39.84 ± 10.40
Vz/F (mL/kg)	343196.86 ± 97272.04	194659.43 ±
CL/F (mL/h/kg)	8236.18 ± 854.77	3326.01 ±
Cmax (ng/mL)	743.76 ± 106.11	1679.54 ±

AUC_0–t, area under the concentration-time curve from 0 to 48 h; AUC_0–∞, area under the concentration-time curve from 0 to time infinite; MRT, mean residence time; t_1/2, half-life of elimination; V_z/F, volume of distribution; CL/F, clearance; C_max, maximum value of concentration; or significant compared to normal rat.