Antioxidant and Neuroproliferative Effects of THL-3-PTD5 Peptide Derived from Hydramacin-1 Antimicrobial Peptide
Table 1
Physicochemical characterization of truncated THL series peptides.
Type
Peptide
Sequence
Theoretical mass (Da)a
Measured mass (Da)b
RP-HPLC (, min)c
Purity (%)d
Truncated peptide of hydramacin-1
THL
RCKELGRKRGQCEEKPSRCPLSKKA-NH2
2887.45
2887.6
14.23
98.3
THL-1
RCKELGRKRGQ-NH2
1329.60
1329.7
12.69
97.8
THL-2
KRGQCEEKPSR-NH2
1316.51
1316.7
12.05
95.6
THL-3
KPSRCPLSKKA-NH2
1213.51
1213.8
13.25
98.6
Alanine-scanning of THL-3
THL-3-A1
APSRCPLSKKA-NH2
1156.41
1157.7
8.74
99.7
THL-3-A2
KASRCPLSKKA-NH2
1187.46
1188.7
10.18
99.9
THL-3-A3
KPARCPLSKKA-NH2
1197.50
1198.8
7.71
99.7
THL-3-A4
KPSACPLSKKA-NH2
1128.39
1129.6
9.01
99.4
THL-3-A5
KPSRAPLSKKA-NH2
1181.43
1179.8
4.95
95.1
THL-3-A6
KPSRCALSKKA-NH2
1187.46
1188.7
6.11
95.7
THL-3-A7
KPSRCPASKKA-NH2
1171.42
1170.2
4.26
92.4
THL-3-A8
KPSRCPLAKKA-NH2
1197.50
1198.0
8.57
99.3
THL-3-A9
KPSRCPLSAKA-NH2
1156.41
1156.8
9.47
99.0
THL-3-A10
KPSRCPLSKAA-NH2
1156.41
1156.8
9.41
99.9
Cell-penetrating
PTD5
YARAARRAARR-NH2
1316.54
1316.5
9.98
99.2
THL-3-PTD5
KPSRCPLSKKA-YARAARRAARR-NH2
2513.02
2513.5
12.10
98.0
aTheoretical molecular weight of the peptides was calculated using the software provided in the website: https://www.bachem.com/knowledge-center/peptide-calculator/. bMolecular weight of peptides were determined by MALDI-TOF MS. cRetention time () was determined by RP-HPLC using the C18 column. dPurity of the peptides was calculated using the following formula on HPLC: peak area of peptide/peak area of all.
