Eugenia supra-axillaris Essential Oil and Its Nanoemulsion: Chemical Characterization, In Vivo Anti-Inflammatory, Analgesic, and Antipyretic Activities

Table 1

Essential oil constituents of the Eugenia supra-axillaris shoots.

No	Rt	Rel. Conc. %^a	Compound name	KI		Type	Identification
				Lit	Cal
1	3.91	24.33 ± 1.12	α-Pinene	931	929	MH	KI, MS
2	4.26	0.18 ± 0.01	Camphene	953	955	MH	KI, MS
3	4.86	1.61 ± 0.03	β-Pinene	964	965	MH	KI, MS
4	5.09	0.38 ± 0.04	α-Myrcene	988	990	MH	KI, MS
5	5.55	0.15 ± 0.01	3-Carene	1011	1013	MH	KI, MS
6	6.14	32.82 ± 2.05	d-Limonene	1022	1024	MH	KI, MS
7	6.64	0.13 ± 0.01	trans-β-Ocimene	1050	1049	MH	KI, MS
8	6.94	0.14 ± 0.01	γ-Terpinene	1057	1055	MH	KI, MS
9	8.28	0.06 ± 0.00	Linalool	1094	1096	OM	KI, MS
10	8.85	0.08 ± 0.00	d-Fenchyl alcohol	1109	1110	OM	KI, MS
11	9.51	0.34 ± 0.02	trans-Pinocarveol	1139	1137	OM	KI, MS
12	9.80	0.23 ± 0.01	Isopulegol	1145	1146	OM	KI, MS
13	10.54	0.18 ± 0.01	Borneol	1149	1149	OM	KI, MS
14	11.11	0.21 ± 0.01	cis-p-Mentha-1(7),8-dien-2-ol	1165	1164	OM	KI, MS
15	11.38	0.58 ± 0.03	α-Terpineol	1169	1171	OM	KI, MS
16	12.65	0.12 ± 0.01	Carveol	1207	1205	OM	KI, MS
17	16.58	0.11 ± 0.00	α-Terpineol acetate	1352	1350	OM	KI, MS
18	16.82	0.54 ± 0.03	α-Ylangene	1375	1376	SH	KI, MS
19	17.29	0.07 ± 0.00	α-Copaene	1374	1372	SH	KI, MS
20	18.22	3.58 ± 0.11	α-Elemene	1388	1487	SH	KI, MS
21	19.06	0.16 ± 0.01	β-Cubebene	1389	1391	SH	KI, MS
22	19.17	3.15 ± 0.07	trans-Caryophyllene	1406	1405	SH	KI, MS
23	19.38	4.73 ± 0.13	α-Humulene	1448	1450	SH	KI, MS
24	20.00	0.48 ± 0.02	γ-Muurolene	1480	1476	SH	KI, MS
25	20.19	4.88 ± 0.12	Germacrene-D	1480	1480	SH	KI, MS
26	20.46	0.14 ± 0.01	β-Selinene	1482	1483	SH	KI, MS
27	20.62	0.64 ± 0.03	α-Muurolene	1491	1490	SH	KI, MS
28	20.75	0.47 ± 0.02	Bicyclogermacrene	1494	1496	SH	KI, MS
29	20.84	0.37 ± 0.01	Eremophilene	1503	1501	SH	KI, MS
30	21.21	0.52 ± 0.02	γ-Cadinene	1510	1514	SH	KI, MS
31	21.33	1.66 ± 0.03	δ-Cadinene	1519	1523	SH	KI, MS
32	23.30	1.05 ± 0.04	Ledol	1565	1567	OS	KI, MS
33	23.43	0.35 ± 0.01	Globulol	1576	1577	OS	KI, MS
34	23.68	0.61 ± 0.02	Caryophyllene oxide	1583	1583	OS	KI, MS
35	23.98	0.13 ± 0.00	Veridiflorol	1590	1589	OS	KI, MS
36	24.16	1.28 ± 0.06	Calarenepoxide	1592	1594	OS	KI, MS
37	24.49	0.12 ± 0.01	Humulene oxide	1605	1607	OS	KI, MS
38	24.65	1.61 ± 0.07	Fonenol	1627	1625	OS	KI, MS
39	24.84	1.45 ± 0.08	Cubenol	1645	1642	OS	KI, MS
40	25.12	1.35 ± 0.06	t-Cadinol	1640	1638	OS	KI, MS
41	25.18	1.88 ± 0.09	t-Muurolol	1640	1641	OS	KI, MS
42	25.52	3.39 ± 0.14	α-Cadinol	1652	1653	OS	KI, MS
43	25.85	0.25 ± 0.01	Khusinol	1658	1661	OS	KI, MS
		96.51	Total
		59.74	Monoterpene hydrocarbons (MH)
		1.91	Oxygenated monoterpenes (OM)
		21.39	Sesquiterpene hydrocarbon (SH)
		13.47	Oxygenated sesquiterpene (OS)

^aValues are the means of three replications (percentage ± standard deviation). Rt, retention time (min); KI Cal, calculated Kováts retention index on DB-5 column in reference to n-alkanes (C8–C22); KI Lit, Kováts retention index from literature.